Aoki Yusuke, Tsujimura Akira, Nagashima Yuki, Hiramatsu Ippei, Uesaka Yuka, Nozaki Taiji, Ogishima Tatsuya, Shirai Masato, Shoyama Yukihiro, Tanaka Hiromitsu, Horie Shigeo
Department of Urology Juntendo University Urayasu Hospital Urayasu Chiba Japan.
Department of Urology Juntendo University Graduate School of Medicine Tokyo Japan.
Reprod Med Biol. 2018 Oct 24;18(1):57-64. doi: 10.1002/rmb2.12251. eCollection 2019 Jan.
The direct effects of (Maca) on sperm remain unclear. Herein, we examined the direct effect of Maca on in vitro fertilization.
We examined the fertilization rate in a mouse model and the rate of acrosome reaction in sperm from transgenic mice expressing enhanced green fluorescent protein (EGFP) in a Maca extract-containing human tubal fluid (HTF) medium. Using human sperm, we assessed acrosome status via fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA) staining and performed detailed analysis using a sperm motility analysis system (SMAS).
In the mouse model, the fertilization rate in the Maca extract-containing HTF was significantly higher than that in the control medium. The acrosome reaction rate in sperm from transgenic mice expressing EGFP was also significantly higher in the Maca extract-containing HTF than that in the control medium. Similarly, a high acrosome reaction rate, identified via FITC-PNA staining of human sperm samples, was found in the Maca extract-containing HTF compared with that in the control medium. Human sperm motility in the Maca extract-containing HTF was also increased compared with that in the control medium as measured using an SMAS.
Maca improved in vitro fertilization rates by inducing an acrosome reaction and increasing sperm motility.
玛咖对精子的直接作用尚不清楚。在此，我们研究了玛咖对体外受精的直接作用。
我们在含玛咖提取物的人类输卵管液（HTF）培养基中，检测了小鼠模型的受精率以及表达增强型绿色荧光蛋白（EGFP）的转基因小鼠精子的顶体反应率。使用人类精子，我们通过异硫氰酸荧光素偶联花生凝集素（FITC-PNA）染色评估顶体状态，并使用精子活力分析系统（SMAS）进行详细分析。
在小鼠模型中，含玛咖提取物的HTF中的受精率显著高于对照培养基。含玛咖提取物的HTF中，表达EGFP的转基因小鼠精子的顶体反应率也显著高于对照培养基。同样，通过对人类精子样本进行FITC-PNA染色发现，含玛咖提取物的HTF中的顶体反应率高于对照培养基。使用SMAS测量发现，含玛咖提取物的HTF中人类精子的活力也高于对照培养基。
玛咖通过诱导顶体反应和增加精子活力提高了体外受精率。
