Zhang Yusen, Wang Guoqiang
Department of Plastic Surgery, People's Hospital of Zhengzhou, Henan Agricultural University, Zhengzhou, Henan 450000, P.R. China.
Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan 450000, P.R. China.
Oncol Lett. 2019 Jan;17(1):548-554. doi: 10.3892/ol.2018.9603. Epub 2018 Oct 22.
To assess the influence of microRNA-183 (miR-183) on the migration and invasion of A375 human melanoma cells, an A375 cell line with stable miR-183 overexpression or knockdown was constructed using lentiviral transfection. The change of miR-183 expression in these cells and in non-transfected controls was verified using reverse transcription-quantitative polymerase chain reaction. The impact of miR-183 on experimental A375 cell migration and invasion was assessed using a scratch and Transwell assay. The expression of Ezrin and matrix metalloprotease-9 (MMP-9), which are two mediator proteins that serve roles in tumor cell migration and invasion, were analyzed in each cell group via western blotting. The results of the present study indicated that miR-183 overexpression significantly inhibits A375 cell migration and invasion, which may be facilitated by miR-183 knockdown. Furthermore, Ezrin and MMP-9 protein levels were negatively associated with miR-183 expression, indicating that miR-183 may function as a tumor suppressor by inhibiting the expression of these two proteins. Additionally, miR-183 downregulation may be associated with the progression of melanoma.
为评估微小RNA-183（miR-183）对A375人黑色素瘤细胞迁移和侵袭的影响，利用慢病毒转染构建了miR-183稳定过表达或敲低的A375细胞系。采用逆转录-定量聚合酶链反应验证这些细胞及未转染对照中miR-183表达的变化。采用划痕试验和Transwell试验评估miR-183对实验性A375细胞迁移和侵袭的影响。通过蛋白质印迹法分析各细胞组中埃兹蛋白（Ezrin）和基质金属蛋白酶-9（MMP-9）的表达，这两种介导蛋白在肿瘤细胞迁移和侵袭中发挥作用。本研究结果表明，miR-183过表达显著抑制A375细胞迁移和侵袭，而miR-183敲低可能会促进这种迁移和侵袭。此外，Ezrin和MMP-9蛋白水平与miR-183表达呈负相关，表明miR-183可能通过抑制这两种蛋白的表达发挥肿瘤抑制作用。此外，miR-183下调可能与黑色素瘤的进展有关。
