重组杀菌肽对肉鸡生长性能、肠道健康和先天免疫应答的影响。
Effects of Dietary Supplementation of Recombinant Plectasin on Growth Performance, Intestinal Health and Innate Immunity Response in Broilers.
机构信息
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China.
National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China.
出版信息
Probiotics Antimicrob Proteins. 2020 Mar;12(1):214-223. doi: 10.1007/s12602-019-9515-2.
The present study was conducted to evaluate the effects of dietary supplementation of recombinant plectasin (Ple) on the growth performance, intestinal health, and serum immune parameters in broilers. A total of 288 1-day-old male broilers (Arbor Acres) were randomly allotted to four dietary treatments including the basal diet (NC) and basal diet supplemented with 10 mg enramycin/kg (PC), 100 mg Ple/kg (LPle), and 200 mg Ple/kg (HPle) diets. The results indicated Ple increased (P < 0.01) average daily gain and decreased (P ≤ 0.02) feed to gain ratio of broilers. In addition, the supplementation of Ple in the diets increased (P ≤ 0.01) duodenal lipase (day 21) and trypsin (day 42) activities compared with the NC group. Similar as the supplementation of enramycin, Ple also increased villus height and decreased crypt depth in jejunum (day 21), and thus the villus height to crypt depth ratio (P < 0.01) was increased compared to the NC group on day 42. The serum immunoglobulin M (days 21 and 42), immunoglobulin G (day 42), complement 3 (day 21), and complement 4 (days 21 and 42) were significantly increased (P ≤ 0.02) due to the supplementation of Ple and enramycin, while the concentration of malondialdehyde in jejunum was decreased (P < 0.01) in PC, LPle, and HPle groups on day 21 compared with those in the NC group. Furthermore, Ple reduced (P < 0.01) Escherichia coli and total aerobic bacteria population in ileum and cecum of birds on days 21 and 42. These results indicate that the recombinant plectasin has beneficial effects on growth performance, intestinal health, and innate immunity in broilers.
本研究旨在评估日粮添加重组聚醚菌素（Ple）对肉鸡生长性能、肠道健康和血清免疫参数的影响。将 288 只 1 日龄雄性肉鸡（Arbor Acres）随机分为 4 个日粮处理组，包括基础日粮（NC）和基础日粮添加 10mg 恩拉霉素/kg（PC）、100mg Ple/kg（LPle）和 200mg Ple/kg（HPle）日粮。结果表明，Ple 提高了肉鸡的平均日增重（P<0.01），降低了料重比（P≤0.02）。此外，日粮中添加 Ple 提高了十二指肠脂肪酶（第 21 天）和胰蛋白酶（第 42 天）活性（P≤0.01），与 NC 组相比。与添加恩拉霉素相似，Ple 还增加了空肠绒毛高度（第 21 天）和降低了隐窝深度（第 21 天），从而增加了绒毛高度与隐窝深度的比值（P<0.01），第 42 天与 NC 组相比。由于添加 Ple 和恩拉霉素，血清免疫球蛋白 M（第 21 天和第 42 天）、免疫球蛋白 G（第 42 天）、补体 3（第 21 天）和补体 4（第 21 天和第 42 天）均显著增加（P≤0.02），而第 21 天 PC、LPle 和 HPle 组空肠丙二醛浓度较 NC 组降低（P<0.01）。此外，Ple 降低了第 21 天和第 42 天肉鸡回肠和盲肠中大肠杆菌和总需氧菌的数量。这些结果表明，重组聚醚菌素对肉鸡的生长性能、肠道健康和先天免疫具有有益的影响。
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