商业有机酸混合物对感染 E. coli K88 的雄性肉鸡的影响：生产性能、微生物学、肠道形态和免疫反应。

Effects of commercial organic acid blends on male broilers challenged with E. coli K88: Performance, microbiology, intestinal morphology, and immune response.

机构信息

Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran, 91779-48974.

Technical Responsible, Shamim Roshd Espadan Co., Sepahan Shahr, Isfahan, Iran; Department of Animal Science, Shahrekord University, Shahrekord, Iran, 88186-34141.

出版信息

Poult Sci. 2017 Sep 1;96(9):3254-3263. doi: 10.3382/ps/pex106.

DOI:10.3382/ps/pex106

PMID:28453753

Abstract

This study assessed the effects of 3 commercial organic acid (OA) preparations on growth performance, intestinal morphology, cecal microbiology, and immunity of Escherichia coli K88-challenged (ETEC) broiler chickens. One thousand one-day-old male broiler chickens were divided into 8 treatments of 5 replicate pens: Negative control (NC) birds received a basal diet (BD) and were not challenged with ETEC; positive control (PC) birds fed the BD and challenged with ETEC; BD + 0.2% (S1) or 0.4% (S2) of an OA mixture (Salkil) from one to 35 d; BD + 0.1, 0.075, and 0.05% (O1) of another OA mixture (Optimax) in the starter (one to 10 d), grower (11 to 24 d), and finisher (25 to 35 d) diets, respectively, or 0.1% (O2) from one to 35 d; BD + 0.07, 0.05, and 0.05% (P1) or 0.1, 0.07, and 0.05% (P2) of a further OA mixture (pHorce) in the starter, grower, and finisher diets, respectively. All groups (not NC) were challenged with one mL of ETEC (1 × 108 cfu/mL) at 7 d of age. The 3 OA mixtures are commercial formic and propionic acid preparations. Birds challenged with ETEC (PC) had reduced (P < 0.05) growth performance, ileal morphological parameters (not crypt depth, which was increased), cecal lactobacilli, and immune responses, and increased cecal E. coli compared with unchallenged, NC birds. The addition of OA to the diets of ETEC challenged birds (S1-P2) either numerically or significantly (P < 0.05) improved growth performance, ileal morphology and immune responses, increased cecal lactobacilli, and reduced cecal E. coli. For most OA additions, the assessed parameters were generally enhanced to equivalence to NC birds. The results suggest that dietary OA supplementation can enhance the growth performance, ileal morphology, cecal microbiota, and immunity of ETEC-challenged broilers to an extent that, under such circumstances, the formulations used in this study provided similar performance and assessed parameters as non-challenged birds.

摘要

本研究评估了 3 种商业有机酸（OA）制剂对大肠杆菌 K88 攻毒（ETEC）肉鸡生长性能、肠道形态、盲肠微生物群和免疫的影响。将 1100 只 1 日龄雄性肉鸡分为 8 个处理组，每组 5 个重复笼：阴性对照组（NC）鸡接受基础日粮（BD），不攻毒 ETEC；阳性对照组（PC）鸡接受 BD 并攻毒 ETEC；BD+0.2%（S1）或 0.4%（S2）的一种 OA 混合物（Salkil）从 1 日龄至 35 日龄；BD+0.1%、0.075%和 0.05%（O1）的另一种 OA 混合物（Optimax）分别在育雏期（1-10 日龄）、生长期（11-24 日龄）和育肥期（25-35 日龄）日粮中，或从 1 日龄至 35 日龄添加 0.1%（O2）；BD+0.07%、0.05%和 0.05%（P1）或 0.1%、0.07%和 0.05%（P2）的另一种 OA 混合物（pHorce）分别在育雏期、生长期和育肥期日粮中。所有组（非 NC 组）在 7 日龄时用 1 mL ETEC（1×108 cfu/mL）攻毒。这 3 种 OA 混合物是商业甲酸和丙酸制剂。与未攻毒的 NC 鸡相比，攻毒 ETEC（PC）的鸡生长性能降低（P<0.05），空肠形态参数（隐窝深度未降低，反而增加）、盲肠乳酸菌和免疫反应降低，盲肠大肠杆菌增加。在 ETEC 攻毒鸡的日粮中添加 OA（S1-P2），无论是数量上还是在统计学上（P<0.05）都提高了生长性能、空肠形态和免疫反应，增加了盲肠乳酸菌，减少了盲肠大肠杆菌。对于大多数 OA 添加物，评估的参数通常提高到与 NC 鸡相当的水平。结果表明，日粮 OA 补充可以提高 ETEC 攻毒肉鸡的生长性能、空肠形态、盲肠微生物群和免疫功能，在这种情况下，本研究中使用的配方提供了与未攻毒鸡相似的性能和评估参数。