Department of Pharmacology, School of Medicine, Faculty of Health Sciences, University of Thessaly, Biopolis, Panepistimiou 3, 41500, Larissa, Greece.
Department of Pharmacology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110, Ioannina, Greece.
Neurochem Res. 2019 Apr;44(4):829-838. doi: 10.1007/s11064-018-02715-y. Epub 2019 Jan 17.
There is scarce information regarding the effects of anesthetic doses of the non-competitive N-methyl-D-aspartate receptor antagonist ketamine on anxiety. The current study evaluated the acute effects of intraperitoneally (i.p.) administered anesthetic ketamine (100 mg/kg) i.p. on anxiety in rats. For this purpose, the light/dark and the open field tests were utilized. The effects of anesthetic ketamine on motility were also examined using a motility cage. In the light/dark test, anesthetic ketamine, administered 24 h before testing reduced the number of transitions between the light and dark compartments and the time spent in the light compartment in the rats compared with their control cohorts. In addition, ketamine was found to exert a depressive effect on rats' motility. In the open field test, animals treated with anesthetic ketamine 24 h before testing spent essentially no time in the central area of the apparatus, decreased horizontal ambulatory activity, and preserved to a certain extent their exploratory behaviour compared to their control counterparts. The results suggest that, in spite of its hypokinetic effect, a single anesthetic ketamine administration apparently induces an anxiety-like state, while largely preserving exploratory behaviour in the rat. These effects were time-dependent they since they were extinguished when testing was carried out 48 h after anesthetic ketamine administration.
关于麻醉剂量的非竞争性 N-甲基-D-天冬氨酸受体拮抗剂氯胺酮对焦虑的影响，相关信息十分有限。本研究评估了腹腔内给予麻醉剂量氯胺酮（100mg/kg）对大鼠焦虑的急性影响。为此，使用了明暗箱和旷场试验。还使用活动箱检查了麻醉氯胺酮对运动的影响。在明暗箱测试中，与对照组相比，在测试前 24 小时给予麻醉氯胺酮可减少大鼠在明暗箱之间的转换次数和在亮箱中的停留时间。此外，氯胺酮对大鼠的运动能力表现出抑制作用。在旷场试验中，与对照组相比，在测试前 24 小时给予麻醉氯胺酮的动物几乎没有在仪器的中央区域停留，水平活动减少，并且在一定程度上保留了它们的探索行为。结果表明，尽管氯胺酮具有抑制运动的作用，但单次给予麻醉氯胺酮显然会引起类似焦虑的状态，而在很大程度上保留了大鼠的探索行为。这些效应是时间依赖性的，因为当在麻醉氯胺酮给药后 48 小时进行测试时，这些效应消失了。
