Ahluwalia Jusleen, Borok Jenna, Haddock Ellen S, Ahluwalia Rahul S, Schwartz Eveie W, Hosseini Dana, Amini Sasan, Eichenfield Lawrence F
Department of Pediatric and Adolescent Dermatology, Rady Children's Hospital, San Diego, California.
Department of Pediatric and Adolescent Dermatology, University of California, San Diego School of Medicine, La Jolla, California.
Pediatr Dermatol. 2019 Mar;36(2):200-206. doi: 10.1111/pde.13741. Epub 2019 Jan 18.
BACKGROUND/OBJECTIVES: The pathogenesis of preadolescent acne has not been well studied, and it is uncertain if Cutibacterium acnes is a predominant organism in the microbiome in this age group. The aim of this study was to analyze the microbiome of preadolescent females and to assess whether benzoyl peroxide impacts the microbiome.
The study enrolled girls, aged 7-12 years, with evidence of at least six acne lesions who had not been previously treated. Participants' skin surface of forehead, cheeks, nose, chin, left retroauricular crease, and extruded contents of a comedonal lesion were sampled at baseline. Participants used benzoyl peroxide 4% wash for 6-8 weeks and returned for skin surface sampling and extraction collection. Microbiome analysis was performed using 16S ribosomal RNA gene amplicon sequencing on all swab and lesional extraction samples.
Fifty-one participants were enrolled with a median IGA score of 2 (mild). Changes in microbiome diversity were associated with increasing age and number of acne lesions (P = 0.001). C. acnes had higher abundances on forehead and nose, as opposed to cheeks and chin (P = 0.009). Bacterial diversity (alpha diversity) of the skin microbiome was comparable between preadolescent at baseline and after treatment with benzoyl peroxide.
This is the first large assessment characterizing female acne microbiome in early and late preadolescence. Results show that preadolescent acne can vary in its microbial profile, reflecting surrounding changes associated with the onset of puberty. Although benzoyl peroxide use was associated with decreased acne counts, its effect on microbial diversity was not demonstrated in our study.
背景/目的：青春期前痤疮的发病机制尚未得到充分研究，目前尚不确定痤疮丙酸杆菌是否为该年龄组微生物群中的主要微生物。本研究的目的是分析青春期前女性的微生物群，并评估过氧化苯甲酰是否会影响微生物群。
该研究招募了7至12岁、有至少6个痤疮皮损且未接受过治疗的女孩。在基线时，采集参与者前额、脸颊、鼻子、下巴、左耳后皱纹处的皮肤表面样本以及一个粉刺皮损的挤出物。参与者使用4%的过氧化苯甲酰洗剂6至8周，之后返回进行皮肤表面采样和提取物收集。对所有拭子和皮损提取物样本进行16S核糖体RNA基因扩增子测序，以进行微生物群分析。
共招募了51名参与者，中位IGA评分为2（轻度）。微生物群多样性的变化与年龄增长和痤疮皮损数量增加相关（P = 0.001）。痤疮丙酸杆菌在前额和鼻子上的丰度较高，而在脸颊和下巴上则相反（P = 0.009）。青春期前女性在基线时和使用过氧化苯甲酰治疗后的皮肤微生物群细菌多样性（α多样性）相当。
这是首次对青春期前早期和晚期女性痤疮微生物群进行的大规模评估。结果表明，青春期前痤疮的微生物特征可能不同，反映了与青春期开始相关的周围变化。虽然使用过氧化苯甲酰与痤疮数量减少有关，但在我们的研究中未证明其对微生物多样性的影响。
