Coughlin Carrie C, Swink Shane M, Horwinski Joseph, Sfyroera Georgia, Bugayev Julia, Grice Elizabeth A, Yan Albert C
Division of Dermatology, Departments of Medicine and Pediatrics, School of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
Philadelphia College of Osteopathic Medicine, Philadelphia, PA, USA.
Pediatr Dermatol. 2017 Nov;34(6):661-664. doi: 10.1111/pde.13261. Epub 2017 Oct 11.
BACKGROUND/OBJECTIVES: Acne, a common pediatric disease, tends to be more comedonal in preadolescents, whereas older individuals are more likely to have inflammatory lesions in addition to comedones. Thus the microbiome of preadolescents may be different. In this pilot study we aimed to characterize the preadolescent acne microbiome, compare the microbiome in preadolescents with and without acne, and investigate changes in the microbiome after topical treatment with benzoyl peroxide or a retinoid in a small cohort of preadolescents.
Participants were 7-10 years of age with (intervention group) or without (control group) acne and were recruited during routine outpatient dermatology visits. Baseline questionnaires, physical examination, and pore strip application were performed for all participants. Intervention group participants were randomized to receive topical therapy with benzoyl peroxide 5% gel or cream or tretinoin 0.025% cream. Participants with acne were followed up 8-10 weeks later and pore strip application was repeated.
Preadolescents with acne were colonized with a greater diversity of cutaneous bacteria than controls and the most commonly identified bacterium was Streptococcus. The number of bacterial species and phylogenetic diversity decreased after treatment with benzoyl peroxide and tretinoin.
The predominant bacteria in microbiome studies of adult acne is Propionibacterium, whereas in this pediatric population we saw a lot of Streptococcus bacteria. After treatment, the microbiomes of intervention group participants more closely resembled those of control group participants.
背景/目的：痤疮是一种常见的儿科疾病，在青春期前儿童中往往以粉刺为主，而年龄较大的个体除粉刺外更易出现炎性皮损。因此，青春期前儿童的微生物群可能有所不同。在这项初步研究中，我们旨在描述青春期前儿童痤疮微生物群的特征，比较有痤疮和无痤疮的青春期前儿童的微生物群，并在一小群青春期前儿童中研究外用过氧化苯甲酰或维甲酸治疗后微生物群的变化。
参与者为7至10岁有痤疮（干预组）或无痤疮（对照组）的儿童，在皮肤科门诊常规就诊时招募。对所有参与者进行基线问卷调查、体格检查和鼻贴采集。干预组参与者随机接受5%过氧化苯甲酰凝胶或乳膏或0.025%维甲酸乳膏的局部治疗。有痤疮的参与者在8至10周后进行随访，并重复鼻贴采集。
与对照组相比，有痤疮的青春期前儿童皮肤细菌的多样性更高，最常鉴定出的细菌是链球菌。用过氧化苯甲酰和维甲酸治疗后，细菌种类数量和系统发育多样性降低。
在成人痤疮微生物群研究中占主导地位的细菌是丙酸杆菌，而在这个儿科人群中我们发现了大量的链球菌。治疗后，干预组参与者的微生物群与对照组参与者的微生物群更相似。
