Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
The Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, China.
Skin Res Technol. 2019 Jul;25(4):424-433. doi: 10.1111/srt.12668. Epub 2019 Jan 18.
The objective of this study was to perform noninvasive analysis of skin proteins in a healthy Chinese population using label-free nanoflow liquid chromatography-mass spectrometry (nLC-MS).
Five consecutive tape strippings were obtained from the volar forearm skin of healthy Chinese subjects. Proteins were extracted, and trypsin-digested peptides were analyzed by a nanochromatography instrument coupled to an Orbitrap Fusion Tribrid mass spectrometer. Data-dependent acquisition allowed protein identification, which was performed by using Proteome Discoverer software (v2.2).
In this study, we identified 80 common proteins that were expressed in the skin of healthy Chinese volunteers and divided these proteins into 16 categories, including keratins, cornified envelope proteins, and enzymes associated with substance metabolism. These proteins were closely associated with multiple functions of the skin barrier.
This study provides a noninvasive method to analyze healthy human epidermal proteins, which are closely associated with the skin barrier. In addition, this study provides a reference for further studies on the application of proteomic technologies to investigate the role of human epidermal proteins in health and disease.
本研究旨在使用无标记纳流液相色谱-质谱联用（nLC-MS）技术对健康中国人群的皮肤蛋白质进行非侵入性分析。
从健康中国受试者的掌侧前臂皮肤连续采集 5 个胶带剥离物。提取蛋白质，用纳色谱仪与轨道阱融合三重四极杆质谱仪分析胰蛋白酶消化肽段。数据依赖型采集允许进行蛋白质鉴定，使用 Proteome Discoverer 软件（v2.2）进行鉴定。
在这项研究中，我们鉴定了 80 种在健康中国志愿者皮肤中表达的常见蛋白质，并将这些蛋白质分为 16 类，包括角蛋白、角化包膜蛋白和与物质代谢相关的酶。这些蛋白质与皮肤屏障的多种功能密切相关。
本研究提供了一种非侵入性的方法来分析与皮肤屏障密切相关的健康人表皮蛋白质。此外，本研究为进一步研究蛋白质组学技术在研究人类表皮蛋白质在健康和疾病中的作用提供了参考。
