Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Mol Med Rep. 2020 May;21(5):2227-2235. doi: 10.3892/mmr.2020.11020. Epub 2020 Mar 11.
A number of epidermal proteins are closely related to skin barrier function, the abnormalities of which can lead to specific skin diseases. These proteins must be quantified to further investigate the changes in the skin barrier between healthy and disease states. However, the non‑invasive and proteome‑wide quantification of skin proteins without any labelling steps remains a challenge. In this study, 3M medical adhesive tapes were used to obtain skin samples from volunteers. Proteins were extracted from fresh skin samples and digested with trypsin. Each tryptic peptide was analysed in three replicates using liquid chromatography with tandem mass spectrometry analysis and label‑free quantification. The data were searched against the Human Universal Protein Resource (UniProt) to match with known proteins. Using this method, 1,157 skin proteins recorded in the UniProt were quantified. A total of 50 identical proteins were identified in the three replicate analyses of all samples with no significant differences in abundance. The results provided an objective metric for further study of skin ageing and various skin diseases. Specifically, the non‑invasive proteome‑wide method used in this study can be applied to future studies of skin diseases related to barrier destruction by monitoring the changes in the levels of epidermal proteins.
许多表皮蛋白与皮肤屏障功能密切相关，其异常可导致特定的皮肤疾病。为了进一步研究健康和疾病状态下皮肤屏障的变化，必须对这些蛋白进行定量分析。然而，在不进行任何标记步骤的情况下，实现非侵入性和蛋白质组范围的皮肤蛋白定量仍然是一个挑战。在这项研究中，使用 3M 医用胶带从志愿者身上获取皮肤样本。从新鲜皮肤样本中提取蛋白质，并用胰蛋白酶进行消化。使用液相色谱串联质谱分析和无标记定量法，在三个重复中分析每个胰蛋白酶肽。将数据与人类通用蛋白质资源（UniProt）进行搜索，以匹配已知蛋白。使用这种方法，在 UniProt 中定量了 1157 种皮肤蛋白。在对所有样本的三个重复分析中，总共鉴定出 50 种相同的蛋白，其丰度没有显著差异。该结果为进一步研究皮肤老化和各种皮肤疾病提供了客观的衡量标准。具体而言，本研究中使用的非侵入性蛋白质组范围的方法可通过监测表皮蛋白水平的变化，应用于未来与屏障破坏相关的皮肤疾病的研究。
