Instituto Agronômico (IAC)-Centro de Grãos e Fibras-Fazenda Santa Elis, Campinas, SP, Brazil.
Centro de Energia Nuclear na Agricultura (CENA)-Av. Centenário, São Dimas-CEP-Piracicaba, SP, Brazil.
PLoS One. 2019 Jan 18;14(1):e0210428. doi: 10.1371/journal.pone.0210428. eCollection 2019.
Common bean is a highly important food in tropical regions, where most production occurs on small farms with limited use of technology and, consequently, greater vulnerability to abiotic stresses such as nutritional stress. Usually phosphorus (P) is the most limiting nutrient for crop growth in these regions. The aim of this study was to characterize the gene expression profiles of the genotypes of common bean IAC Imperador (P-responsive) and DOR 364 (P-unresponsive) under different P concentrations using RNA-seq transcriptome sequencing technology. Plants were grown hydroponically, with application of two P concentrations (4.00 mg L-1 restrictive level and 8.00 mg L-1 control level). Differential expression analyses, annotation, and functional classification were performed comparing genotypes within each P rate administered and comparing each genotype response to the different P levels. Considering differential expression analyses within genotypes, IAC Imperador exhibited 1538 up-regulated genes under P restriction and 1679 up-regulated genes in the control, while DOR 364 exhibited 13 up-regulated genes in the control and only 2 up-regulated genes under P restriction, strongly corroborating P-unresponsiveness of this genotype. Genes related to phosphorus restriction were identified among the differentially expressed genes, including transcription factors such as WRKY, ERF, and MYB families, phosphatase related genes such as pyrophosphatase, acid phosphatase, and purple acid phosphatase, and phosphate transporters. The enrichment test for the P restriction treatment showed 123 enriched gene ontologies (GO) for IAC Imperador, while DOR 364 enriched only 24. Also, the enriched GO correlated with P metabolism, compound metabolic processes containing phosphate, nucleoside phosphate binding, phosphorylation, and also response to stresses. Thus, this study proved to be informative to phosphorus limitation in common bean showing global changes at transcript level.
菜豆是热带地区非常重要的食物，大多数菜豆生产都是在小农户中进行的，他们使用的技术有限，因此更容易受到非生物胁迫的影响，如营养胁迫。通常情况下，在这些地区，磷（P）是限制作物生长的最主要营养元素。本研究旨在利用 RNA-seq 转录组测序技术，对响应磷（P）的 IAC Imperador 基因型和不响应 P 的 DOR 364 基因型在不同 P 浓度下的基因表达谱进行特征描述。采用水培法种植植物，施加两种 P 浓度（4.00 mg L-1 为限制浓度，8.00 mg L-1 为对照浓度）。对每个 P 浓度下的基因型进行差异表达分析、注释和功能分类，并比较每个基因型对不同 P 水平的响应。考虑到基因型内的差异表达分析，IAC Imperador 在 P 限制下有 1538 个上调基因，在对照中有 1679 个上调基因，而 DOR 364 在对照中有 13 个上调基因，在 P 限制下只有 2 个上调基因，强烈证实了该基因型对 P 的不响应性。在差异表达基因中鉴定出与磷限制相关的基因，包括 WRKY、ERF 和 MYB 家族等转录因子、焦磷酸酶、酸性磷酸酶和紫色酸性磷酸酶等磷酸酶相关基因以及磷酸盐转运体。对 P 限制处理的富集测试表明，IAC Imperador 有 123 个富集的基因本体（GO），而 DOR 364 仅富集了 24 个。此外，富集的 GO 与 P 代谢、含磷酸盐的化合物代谢过程、核苷磷酸盐结合、磷酸化以及应激反应相关。因此，本研究在菜豆磷限制方面提供了有价值的信息，显示了转录水平的全局变化。
