López Cristina María, Pineda Manuel, Alamillo Josefa M
Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas, Campus de Excelencia Internacional Agroalimentario, CEIA3, Campus de Rabanales, Edif. Severo Ochoa, Universidad de Córdoba, 1407 Córdoba, Spain.
Plants (Basel). 2020 Apr 2;9(4):445. doi: 10.3390/plants9040445.
Drought is one of the most critical factors limiting legume crop productivity. Understanding the molecular mechanisms of drought tolerance in the common bean is required to improve the yields of this important crop under adverse conditions. In this work, RNA-seq analysis was performed to compare the transcriptome profiles of drought-stressed and well-irrigated plants of a previously characterized drought-tolerant common bean landrace. The analysis revealed responses related with the abscisic acid signaling, including downregulation of a phosphatase 2C (PP2C) and an abscisic acid-8' hydroxylase, and upregulation of several key transcription factors and genes involved in cell wall remodeling, synthesis of osmoprotectants, protection of photosynthetic apparatus, and downregulation of genes involved in cell expansion. The results also highlighted a significant proportion of differentially expressed genes related to phosphate starvation response. In addition, the moderate detrimental effects of drought in the biomass of these tolerant plants were abolished by the addition of phosphate, thus indicating that, besides the ABA-mediated response, acquisition of phosphate could be crucial for the drought tolerance of this common bean genotype. These results provided information about the mechanisms involved in drought response of common bean response that could be useful for enhancing the drought tolerance of this important crop legume.
干旱是限制豆类作物产量的最关键因素之一。为了在不利条件下提高这种重要作物的产量，需要了解普通菜豆耐旱性的分子机制。在这项研究中，我们进行了RNA测序分析，以比较一个先前已鉴定的耐旱普通菜豆地方品种在干旱胁迫和充分灌溉条件下的转录组图谱。分析揭示了与脱落酸信号传导相关的反应，包括一个磷酸酶2C（PP2C）和一个脱落酸-8'羟化酶的下调，以及几个关键转录因子和参与细胞壁重塑、渗透保护剂合成、光合装置保护的基因的上调，和参与细胞扩张的基因的下调。结果还突出了相当一部分与磷饥饿反应相关的差异表达基因。此外，添加磷消除了干旱对这些耐旱植物生物量的中度有害影响，因此表明，除了脱落酸介导的反应外，获取磷对于这种普通菜豆基因型的耐旱性可能至关重要。这些结果提供了有关普通菜豆干旱反应机制的信息，这可能有助于提高这种重要豆类作物的耐旱性。
