Regenerative Engineering & Materials, Department of Biomedical Engineering, Eindhoven University of Technology, the Netherlands.
Amsterdam UMC, University of Amsterdam, Biomedical Engineering and Physics, Meibergdreef 9, Amsterdam, the Netherlands.
J Mech Behav Biomed Mater. 2019 Apr;92:50-57. doi: 10.1016/j.jmbbm.2018.12.026. Epub 2018 Dec 21.
Pressure-induced deep tissue injury is a form of pressure ulcer which is difficult to detect and diagnose at an early stage, before the wound has severely progressed and becomes visible at the skin surface. At the present time, no such detection technique is available. To test the hypothesis that muscle damage biomarkers can be indicative of the development of deep tissue injury after sustained mechanical loading, an indentation test was performed for 2 h on the tibialis anterior muscle of rats. Myoglobin and troponin were analysed in blood plasma and urine over a period of 5 days. The damage as detected by the biomarkers was compared to damage as observed with T MRI to validate the response. We found that myoglobin and troponin levels in blood increased due to the damage. Myoglobin was also increased in urine. The amount of damage observed with MRI immediately after loading had a strong correlation with the maximal biomarker levels: troponin in blood r = 0.94; myoglobin in blood r = 0.75; and myoglobin in urine r = 0.57. This study suggests that muscle damage markers measured in blood and urine could serve as early diagnosis for pressure induced deep tissue injury.
压力性深部组织损伤是一种压力性溃疡，在伤口严重进展并在皮肤表面可见之前，很难在早期发现和诊断。目前，还没有这样的检测技术。为了验证肌肉损伤生物标志物是否可以预示持续机械负荷后深部组织损伤的发展，对大鼠的胫骨前肌进行了 2 小时的压痕试验。在 5 天的时间里，分析了血浆和尿液中的肌红蛋白和肌钙蛋白。通过与 T 磁共振成像 (MRI) 进行比较，对生物标志物检测到的损伤进行了验证。我们发现，由于损伤，血液中的肌红蛋白和肌钙蛋白水平升高。肌红蛋白也在尿液中增加。加载后立即进行 MRI 观察到的损伤量与最大生物标志物水平有很强的相关性：血液中的肌钙蛋白 r = 0.94；血液中的肌红蛋白 r = 0.75；尿液中的肌红蛋白 r = 0.57。这项研究表明，血液和尿液中测量的肌肉损伤标志物可作为压力性深部组织损伤的早期诊断。
