Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Arch Oral Biol. 2019 Mar;99:113-119. doi: 10.1016/j.archoralbio.2019.01.006. Epub 2019 Jan 11.
The aim of this study was to investigate the antifungal activities of cell-free supernatants of a probiotic strain, Pediococcus acidilactici HW01, against Candida albicans.
C. albicans was cultured in the presence of different concentration of cell-free supernatants obtained from P. acidilactici HW01 (HW01 CFS) and the growth of C. albicans was determined. C. albicans was incubated with HW01 CFS for 24 h and the biofilm formation of C. albicans was determined by staining crystal violet and by using a scanning electron microscope. Biofilm quantification was determined by 2, 3-Bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay.
HW01 CFS inhibitedC. albicans growth, whereas bacteriocin, which is a well-known antimicrobial peptide of lactic acid bacteria, failed to inhibit C. albicans growth. Pre-treatment and simultaneous treatment with HW01 CFS exhibited a significant inhibition of C. albicans biofilm. Although post-treatment with HW01 CFS did not disrupt the established biofilm of C. albicans at 3 h-incubation, significant reduced C. albicans biofilm was observed after 6 h-incubation in the presence of HW01 CFS.
These results suggested that the CFS fromP. acidilactici HW01 was revealed as an effective antifungal agent against C. albicans by reducing the growth and biofilm formation.
本研究旨在探究一株益生菌——戊糖片球菌 HW01 的无细胞上清液对白色念珠菌的抗真菌活性。
将不同浓度的戊糖片球菌 HW01 的无细胞上清液（HW01 CFS）加入到白色念珠菌的培养液中，检测白色念珠菌的生长情况。将白色念珠菌与 HW01 CFS 孵育 24 h，通过结晶紫染色和扫描电子显微镜观察白色念珠菌生物膜的形成。通过 2,3-双（2-甲氧基-4-硝基-5-磺苯基）-2H-四唑-5-羧基苯胺（XTT）测定法来确定生物膜的定量。
HW01 CFS 抑制了白色念珠菌的生长，而细菌素，这是一种众所周知的乳酸菌抗菌肽，未能抑制白色念珠菌的生长。HW01 CFS 的预处理和同时处理对白色念珠菌生物膜表现出显著的抑制作用。虽然 HW01 CFS 的后处理在 3 h 孵育时没有破坏白色念珠菌已建立的生物膜，但在 6 h 孵育时，HW01 CFS 存在时，明显减少了白色念珠菌生物膜的形成。
这些结果表明，HW01 戊糖片球菌的 CFS 通过减少白色念珠菌的生长和生物膜的形成，显示出对白色念珠菌的有效抗真菌作用。
