Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas No. 800, col Colinas de la Normal, C.P. 44270, Guadalajara, Jalisco, Mexico.
Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Av. General Ramón Corona No. 2514, Col. Nuevo México, Jalisco, C.P. 45138, Zapopan, Mexico.
Plant Foods Hum Nutr. 2022 Jun;77(2):212-219. doi: 10.1007/s11130-022-00966-3. Epub 2022 Apr 23.
There is great interest in the search for new alternatives to antimicrobial drugs, and the use of prebiotics and probiotics is a promising approach to this problem. This study aimed to assess the effect of inulin-type fructans, used in synbiotic combinations with Lactobacillus paracasei or Lactobacillus plantarum, on the production of short-chain fatty acids and antimicrobial activity against Candida albicans. The inhibition assay using the L. paracasei and L. plantarum supernatants resulting from the metabolization of inulin-type fructans displayed growth inhibition and antibiofilm formation against C. albicans. Inhibition occurred at concentrations of 12.5, 25, and 50% of the L. paracasei supernatant and at a concentration of 50% of the L. plantarum supernatant. The analysis of short-chain fatty acids by gas chromatography showed that lactic acid was the dominating produced metabolite. However, acetic, propionic, and butyric acids were also detected in supernatants from both probiotics. Therefore, the synbiotic formulation of L. paracasei or L. plantarum in the presence of inulin-type fructans constitutes with anticandidal effect is a possible option to produce antifungal drugs or antimicrobial compounds.
人们对寻找新型抗菌药物替代品非常感兴趣，而使用益生元和益生菌是解决这个问题的一种有前途的方法。本研究旨在评估菊粉型果聚糖与副干酪乳杆菌或植物乳杆菌联合使用作为共生体对短链脂肪酸产生和抗白色念珠菌抗菌活性的影响。使用菊粉型果聚糖代谢产生的副干酪乳杆菌和植物乳杆菌上清液进行的抑制试验显示出对白色念珠菌的生长抑制和抗生物膜形成作用。在 12.5%、25%和 50%的副干酪乳杆菌上清液浓度以及 50%的植物乳杆菌上清液浓度下发生抑制。通过气相色谱分析短链脂肪酸表明，乳酸是主要产生的代谢物。然而，在两种益生菌的上清液中也检测到了乙酸、丙酸和丁酸。因此，在菊粉型果聚糖存在下的副干酪乳杆菌或植物乳杆菌共生体配方具有抗真菌作用，是生产抗真菌药物或抗菌化合物的一种可能选择。
