Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect in Poultry Feed Samples.

Fumonisins are a group of toxic secondary metabolites that are produced by which are associated with poultry health hazard and great economic losses. The objective of the present study was to develop an immunological method to detect in poultry feed samples. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a polyclonal antibody against 67 kDa protein of the 97K exoantigen was developed to detect this fungus. Antibody anti-67 kDa protein showed cross-reactivity against (2⁻7%) and (10%), but no or low cross-reactivity against sp. and sp. exoantigens. The detection limit for the 67 kDa protein of was 29 ng/mL. Eighty-one poultry feed samples were analyzed for sp. count, 67 kDa protein of and fumonisin concentrations. Eighty of the 81 feed samples (98.6%) showed sp. contamination (mean 6.2 x 10⁴ CFU/g). Mean 67 kDa protein and fumonisin concentration in the poultry feed samples was 21.0 µg/g and 1.02 µg/g, respectively. The concentration of 67 kDa protein, as determined by ic-ELISA correlated positively (p < 0.05) with fumonisin levels (r = 0.76). These results suggest that this ic-ELISA has potential to detect and predict fumonisin contamination in poultry feed samples.