Ono Elisabete Y S, Fungaro Maria Helena P, Sofia Silvia Helena, Figueira Edson L Z, Gerage Antonio Carlos, Ichinoe Masakatsu, Sugiura Yoshitsugu, Ueno Y, Hirooka Elisa Y
Department of Biochemistry and Biotechnology, Center of Exact Sciences, State University of Londrina, PO Box 6001, 86051-990, Londrina, Paraná, Brazil.
Mycopathologia. 2004 Nov;158(4):451-5. doi: 10.1007/s11046-004-3863-4.
Eleven feed samples associated with six animal (horse and poultry) intoxication outbreaks (1991) in the state of Paraná, Brazil, were evaluated for fungal and fumonisin contamination. In order to estimate the trend of livestock intoxication, fumonisin contamination was monitored in corn produced both at the commercial level (1991, 1995 crop), and in an experimental field at a local Agronomy Institute (1997 crop). The total mould count in the feed samples ranged from 2.9 x 10(3) to 1.9 x 10(7) CFU/g, with Fusarium verticillioides as the predominant species, at a high count of 2.4 x 10(4)-6.5 x 10(5) CFU/g. Fumonisins (FB1 + FB2) were detected in all corn-based feed samples at levels ranging from 2.89 to 14.54 microg/g. All 27 Northern corn samples (1991 crop) were contaminated with fumonisins at levels ranging from 2.32 to 16.64 microg/g. Twenty-six (96.3%) out of 27 corn samples from the Central-Southern region (1995 crop) were positive for fumonisins (FB1+FB2), with the range of 0.07-3.66 microg/g, while all 37 Northern samples (1995 crop) were contaminated with fumonisins ranging from 0.57 to 9.97 microg/g. Twenty-one out of 37 corn samples from the Northern region (1997 crop) were positive for fumonisins, but at low level (range of 0.05-2.67 microg/g). The results showed a decreasing trend in fumonisin contamination over the years. Nowadays animal intoxication outbreaks rarely occur in this State, as both animal producers and feed industries have become conscious about monitoring of corn and other raw materials at the quality control level.
对巴西巴拉那州1991年与6起动物（马和家禽）中毒事件相关的11份饲料样本进行了真菌和伏马毒素污染评估。为了估计牲畜中毒趋势，对商业层面生产的玉米（1991年、1995年作物）以及当地农学院试验田种植的玉米（1997年作物）中的伏马毒素污染情况进行了监测。饲料样本中的霉菌总数在2.9×10³至1.9×10⁷CFU/g之间，其中轮枝镰孢菌为优势菌种，含量较高，为2.4×10⁴至6.5×10⁵CFU/g。在所有以玉米为基础的饲料样本中均检测到伏马毒素（FB1 + FB2），含量范围为2.89至14.54μg/g。所有27份北方玉米样本（1991年作物）均被伏马毒素污染，含量范围为2.32至16.64μg/g。中南地区27份玉米样本（1995年作物）中有26份（96.3%）伏马毒素（FB1 + FB2）呈阳性，含量范围为0.07至3.66μg/g，而所有37份北方样本（1995年作物）均被伏马毒素污染，含量范围为0.57至9.97μg/g。北方地区37份玉米样本（1997年作物）中有21份伏马毒素呈阳性，但含量较低（范围为0.05至2.67μg/g）。结果显示，多年来伏马毒素污染呈下降趋势。如今，该州很少发生动物中毒事件，因为动物生产者和饲料行业都已意识到在质量控制层面监测玉米和其他原材料的重要性。
