Department of Food Science and Technology, Food Chemistry Laboratory, BOKU - University of Natural Resources and Life Sciences, Vienna, Muthgasse 11, A-1190 Vienna, Austria.
Department of Food Science and Technology, Food Chemistry Laboratory, BOKU - University of Natural Resources and Life Sciences, Vienna, Muthgasse 11, A-1190 Vienna, Austria.
Food Chem. 2019 May 30;281:124-129. doi: 10.1016/j.foodchem.2018.12.039. Epub 2018 Dec 15.
Insects are rich in major nutrients, such as protein and fat. Recently, minor nutrients like vitamins have become the subjects of interest in insects. Hence, this study reports on the development and validation of a method for the determination of vitamin B in mealworm (Tenebrio molitor larvae), cricket (Gryllus assimilis), grasshopper (Locusta migratoria) and cockroach (Shelfordella lateralis), using an ultra-high performance liquid chromatography approach with preliminary immunoaffinity chromatography sample preparation. The method was validated regarding linearity, specificity, accuracy and precision, as well as limits of detection/quantification, and was found to be satisfactory for the desired application. Found levels of vitamin B were 1.08 µg/100 g for mealworm, 2.88 µg/100 g for cricket, 0.84 µg/100 g for grasshopper, and 13.2 µg/100 g dry weight for cockroach, representing the first validated report on the content of vitamin B in edible insects. Observed interferences are likely caused by the presence of pseudovitamin B.
昆虫富含主要营养素，如蛋白质和脂肪。最近，维生素等微量营养素也成为了昆虫研究的热点。因此，本研究建立并验证了一种利用超高效液相色谱法，结合免疫亲和色谱前处理方法测定黄粉虫（Tenebrio molitor 幼虫）、蟋蟀（Gryllus assimilis）、蝗虫（Locusta migratoria）和蟑螂（Shelfordella lateralis）中维生素 B 的方法。该方法对线性、特异性、准确性和精密度，以及检测限/定量限进行了验证，结果表明该方法适用于预期的应用。检测到的维生素 B 含量分别为黄粉虫 1.08μg/100g、蟋蟀 2.88μg/100g、蝗虫 0.84μg/100g、蟑螂 13.2μg/100g 干重，这是首次对食用昆虫中维生素 B 含量的验证报告。观察到的干扰可能是由于存在类维生素 B 所致。
