The United Graduate School of Agricultural Sciences, Tottori University, Tottori 680-8553, Japan.
Department of Agricultural, Life, and Environmental Sciences, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
Food Chem. 2021 Jun 15;347:129048. doi: 10.1016/j.foodchem.2021.129048. Epub 2021 Jan 12.
In this study, we determined the vitamin B content of commercially-available edible insect products using a bioassay based on Lactobacillus delbrueckii ATCC 7830. Although the vitamin content of giant water bug, bee larva, grasshopper, and weaver ant products was low, we found that diving beetle and cricket products contained relatively high amounts of vitamin B (approximately 89.5 and 65.8 µg/100 g dry weight, respectively). In the cricket products most widely circulated as foods, specific corrinoid (vitamin B) compounds were extracted and identified using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Despite the bioassay detecting high vitamin B content (approximately 50-75 µg/100 g dry weight) in these cricket products, UPLC-MS/MS analysis indicated that pseudovitamin B and 2-methylmercaptoadenyl cobamide (also known as factor S) were actually the predominant corrinoid compounds (~74% and ~21%, respectively), with authentic vitamin B making up only 5% of total corrinoids.
在这项研究中，我们使用基于 Lactobacillus delbrueckii ATCC 7830 的生物测定法来确定市售可食用昆虫产品中的维生素 B 含量。虽然大水蝽、蜂幼虫、蝗虫和织工蚁产品的维生素含量较低，但我们发现龙虱和蟋蟀产品含有相对较高量的维生素 B（分别约为 89.5 和 65.8 µg/100 g 干重）。在作为食品最广泛流通的蟋蟀产品中，使用超高效液相色谱-串联质谱法（UPLC-MS/MS）提取并鉴定了特定的类咕啉（维生素 B）化合物。尽管生物测定法检测到这些蟋蟀产品中含有高维生素 B 含量（约 50-75 µg/100 g 干重），但 UPLC-MS/MS 分析表明，假维生素 B 和 2-甲基巯基腺嘌呤 cobamide（也称为因子 S）实际上是主要的类咕啉化合物（分别约为 74%和 21%），而真正的维生素 B 仅占总类咕啉的 5%。
