State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Jiangxi Province Center for Disease Control and Prevention, Nanchang 330047, China.
J Dairy Sci. 2019 Mar;102(3):1887-1900. doi: 10.3168/jds.2018-15462. Epub 2019 Jan 17.
Dairy-related food safety outbreaks, such as food-borne pathogen contamination, mycotoxin contamination, and veterinary drug contamination, sometimes happen and have been reported all over the world, affecting human health and, in some cases, leading to death. Thus, rapid yet robust detection methods are needed to monitor milk and milk powder for the presence of hazardous substances. The lateral flow immunoassay (LFI) is widely used in onsite testing because of its rapidity, simplicity, and convenience. In this review, we describe some traditional LFI used to detect hazardous substances in milk and milk powder. Furthermore, we discuss recent advances in LFI that aim to improve sensitivity or detection efficiency. These advances include the use of novel label materials, development of signal amplification systems, design of multiplex detection systems, and the use of nucleic acid-based LFI.
乳制品相关的食品安全问题时有发生，例如食源性致病菌污染、真菌毒素污染和兽药污染等，这些问题在世界范围内都有报道，影响了人类健康，在某些情况下甚至导致了死亡。因此，需要快速而强大的检测方法来监测牛奶和奶粉中有害物质的存在。由于其快速、简单和方便，侧向流动免疫分析（LFI）在现场测试中得到了广泛应用。在本文综述中，我们描述了一些传统的 LFI 用于检测牛奶和奶粉中有害物质的方法。此外，我们还讨论了旨在提高灵敏度或检测效率的 LFI 的最新进展。这些进展包括新型标记材料的应用、信号放大系统的开发、多重检测系统的设计以及核酸 LFI 的应用。
