School of Forest Sciences, University of Eastern Finland, Yliopistokatu 7, P.O. Box 111, 80100 Joensuu, Finland.
School of Forest Sciences, University of Eastern Finland, Yliopistokatu 7, P.O. Box 111, 80100 Joensuu, Finland.
Ecotoxicol Environ Saf. 2019 Apr 30;171:753-770. doi: 10.1016/j.ecoenv.2019.01.045. Epub 2019 Jan 17.
This study was conducted to evaluate the effects of lime and bisphosphonates (BPs) such as N10O chelate amendment on the growth, physiological and biochemical parameters, and phytoextraction potential of the willow variety Klara (Salix viminalis × S. schwerinii × S. dasyclados) grown in soils heavily contaminated with copper (Cu), nickel (Ni) and zinc (Zn). The plants were irrigated with tap or processed water (mine wastewater). The results suggest that the combined effects of the contaminated soil and processed water inhibited growth parameters, gas exchange parameters and chlorophyll fluorescence (Fv/Fm) values. In contrast, malondialdehyde (MDA) content, organic acids, total phenolic and total flavonoid contents, and the accumulation of metals/metalloids in the plant tissues were increased compared to the control. When the soil was supplemented with lime and N10O; growth, physiological, biochemical parameters, and resistance capacity were significantly higher compared to unamended soil treatments, especially in the contaminated soil treatments. The combined lime‒ and N10O‒amended soil treatment produced higher growth rates, resistance capacity, photosynthesis rates and phytoextraction efficiency levels relative to either the lime‒amended or the N10O‒amended soil treatments. This study provides practical evidence of the efficient chelate‒assisted phytoextraction capability of Klara and highlights its potential as a viable and inexpensive novel approach for in situ remediation of Cu‒, Ni‒ and Zn‒contaminated soils and mine wastewaters.
本研究旨在评估石灰和双膦酸盐（BPs）（如 N10O 螯合剂）对在铜（Cu）、镍（Ni）和锌（Zn）重度污染土壤中生长的柳树品种 Klara（柳属 × 柳属 × 柳属）的生长、生理和生化参数以及植物提取潜力的影响。植物用自来水或加工水（矿山废水）灌溉。结果表明，受污染土壤和加工水的联合作用抑制了生长参数、气体交换参数和叶绿素荧光（Fv/Fm）值。相比之下，与对照相比，丙二醛（MDA）含量、有机酸、总酚和总类黄酮含量以及植物组织中金属/类金属的积累增加。当土壤中补充石灰和 N10O 时，与未改良土壤处理相比，生长、生理、生化参数和抗逆能力显著提高，尤其是在受污染土壤处理中。与单独施用石灰或 N10O 相比，石灰和 N10O 联合改良土壤处理的生长速率、抗逆能力、光合作用速率和植物提取效率更高。本研究为 Klara 高效螯合辅助植物提取能力提供了实际证据，并强调了其作为一种可行且廉价的新型原位修复 Cu-、Ni-和 Zn-污染土壤和矿山废水的方法的潜力。
