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一种新型可生物降解和温敏性聚（酯酰胺）水凝胶用于软骨组织工程。

A Novel Biodegradable and Thermosensitive Poly(Ester-Amide) Hydrogel for Cartilage Tissue Engineering.

机构信息

Department of Orthopaedic Surgery, Chang Gung Memorial Hospital in Keelung, Chang Gung University, School of Medicine, Taoyuan, Taiwan.

Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan.

出版信息

Biomed Res Int. 2018 Dec 19;2018:2710892. doi: 10.1155/2018/2710892. eCollection 2018.

DOI:10.1155/2018/2710892
PMID:30662902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6313982/
Abstract

Thermosensitive hydrogels are attractive alternative scaffolding materials for minimally invasive surgery through a simple injection and in situ gelling. In this study, a novel poly(ester-amide) polymer, methoxy poly(ethylene glycol)-poly(pyrrolidone-co-lactide) (mPDLA, P3L7) diblock copolymer, was synthesized and characterized for cartilage tissue engineering. A series of amphiphilic diblock copolymers was synthesized by ring-opening polymerization of mPEG 550, D,L-lactide, and 2-pyrrolidone. By dynamic light scattering analysis and tube-flipped-upside-down method, viscoelastic properties of the mPDLA diblock copolymer solution exhibited sol-gel transition behavior as a function of temperature. An in vitro degradation assay showed that degradation acidity was effectively reduced by introducing the 2-pyrrolidone monomer into the polyester hydrogel. Besides, mPDLA exhibited great biocompatibility in vitro for cell encapsulation due to a high swelling ratio. Moreover, cell viability and biochemical analysis proved that the mPDLA hydrogel presented a great chondrogenic response. Taken together, these results demonstrate that mPDLA hydrogels are promising injectable scaffolds potentially applicable to cartilage tissue engineering.

摘要

温敏水凝胶是一种有吸引力的替代支架材料，可通过简单的注射和原位凝胶化用于微创外科手术。在这项研究中，合成了一种新型聚（酯酰胺）聚合物，甲氧基聚乙二醇-聚（吡咯烷酮-co-丙交酯）（mPDLA，P3L7）两亲性嵌段共聚物，用于软骨组织工程。通过 mPEG550、D，L-丙交酯和 2-吡咯烷酮的开环聚合合成了一系列两亲性嵌段共聚物。通过动态光散射分析和管翻转倒置法，mPDLA 嵌段共聚物溶液的粘弹性性质表现出作为温度函数的溶胶-凝胶转变行为。体外降解实验表明，通过将 2-吡咯烷酮单体引入聚酯水凝胶中，可以有效降低降解酸度。此外，由于高溶胀比，mPDLA 表现出良好的体外细胞包封相容性。此外，细胞活力和生化分析证明 mPDLA 水凝胶具有良好的软骨生成反应。综上所述，这些结果表明 mPDLA 水凝胶是一种有前途的可注射支架，可能适用于软骨组织工程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/bb86ed891b6c/BMRI2018-2710892.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/778086a01815/BMRI2018-2710892.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/e40b43f4c7d0/BMRI2018-2710892.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/da5d08f2e407/BMRI2018-2710892.003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/665dfe7ebc3d/BMRI2018-2710892.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/3e3af7913945/BMRI2018-2710892.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/c493f2a4a7f0/BMRI2018-2710892.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/7f432fbc396f/BMRI2018-2710892.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/bb86ed891b6c/BMRI2018-2710892.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/778086a01815/BMRI2018-2710892.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/e40b43f4c7d0/BMRI2018-2710892.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/da5d08f2e407/BMRI2018-2710892.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/bb95a40c25c5/BMRI2018-2710892.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/665dfe7ebc3d/BMRI2018-2710892.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/3e3af7913945/BMRI2018-2710892.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/c493f2a4a7f0/BMRI2018-2710892.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/7f432fbc396f/BMRI2018-2710892.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2b7/6313982/bb86ed891b6c/BMRI2018-2710892.009.jpg

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