State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China.
J Phys Chem B. 2009 Dec 31;113(52):16518-25. doi: 10.1021/jp907974d.
In this study, we synthesized a biodegradable triblock copolymer poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) by ring-opening copolymerization, and nanohydroxyapatite (n-HA) powder was prepared by a hydrothermal precipitation method. The obtained n-HA was incorporated into the PECE matrix to prepare injectable thermosensitive hydrogel nanocomposites. (1)H NMR, FT-IR, XRD, DSC, and TEM were used to investigate the properties of PECE copolymer and n-HA/PECE nanocomposites. The rheological measurements for n-HA/PECE nanocomposites revealed that the gelation temperature was approximately 36 degrees C. The sol-gel-sol transition behavior and phase transition diagrams were recorded through a test tube inverting method. The results showed that n-HA/PECE nanocomposites still had thermoresponsivity like that of PECE thermosensitive hydrogel. The morphology of the nanocomposites was observed by SEM; the results showed that the nanocomposites had a 3D network structure. In addition, the effects of n-HA contents on the properties of n-HA/PECE nanocomposites are also discussed in the paper. From the results, n-HA/PECE hydrogel is believed to be promising for injectable orthopedic tissue engineering due to its good thermosensitivity and injectability.
在这项研究中，我们通过开环共聚合成了一种可生物降解的三嵌段共聚物聚乙二醇-聚己内酯-聚乙二醇（PEG-PCL-PEG，PECE），并通过水热沉淀法制备了纳米羟基磷灰石（n-HA）粉末。将得到的 n-HA 掺入到 PECE 基质中，制备了可注射的温敏水凝胶纳米复合材料。通过（1）H NMR、FT-IR、XRD、DSC 和 TEM 研究了 PECE 共聚物和 n-HA/PECE 纳米复合材料的性能。对 n-HA/PECE 纳米复合材料的流变学测量表明，凝胶温度约为 36 摄氏度。通过试管倒置法记录了溶胶-凝胶-溶胶转变行为和相转变图。结果表明，n-HA/PECE 纳米复合材料仍具有与 PECE 温敏水凝胶相似的温敏性。通过 SEM 观察纳米复合材料的形态，结果表明纳米复合材料具有 3D 网络结构。此外，本文还讨论了 n-HA 含量对 n-HA/PECE 纳米复合材料性能的影响。从结果来看，n-HA/PECE 水凝胶由于其良好的温敏性和可注射性，有望应用于可注射的骨科组织工程。
