Al-Shehri Hajri, James LaCourse E, Klimach Otto, Kabatereine Narcis B, Stothard J Russell
Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
Ministry of Health, Asir District, Kingdom of Saudi Arabia.
Parasite Epidemiol Control. 2018 Nov 23;4:e00074. doi: 10.1016/j.parepi.2018.e00074. eCollection 2019 Feb.
As part of an epidemiological survey for gastrointestinal parasites in school children across five primary schools on the shoreline of Lake Albert, the prevalence of giardiasis was 87.0% (n = 254) as determined by real-time PCR analysis of faecal samples with a genus-specific 18S rDNA probe. Faecal samples were further characterised with taxon assemblage-specific triose phosphate isomerase (TPI) Taqman® probes and by sequence characterisation of the -giardin gene. While less sensitive than the 18S rDNA assay, general prevalence by TPI probes was 52.4%, with prevalence by taxon assemblage of 8.3% (assemblage A), 35.8% (assemblage B) and 8.3% co-infection (A & B assemblages). While assemblage B was dominant across the sample, proportions of assemblages A and B, and co-infections thereof, varied by school and by age of child; mixed infections were particularly common at Runga school (OR = 6.9 [95% CI; 2.5, 19.3]) and in children aged 6 and under (OR = 2.7 [95% CI; 1.0, 7.3]). Infection with assemblage B was associated with underweight children (OR = 2.0 [95% CI; 1.0, 3.9]). The presence of each assemblage was also confirmed by sequence analysis of the -giardin gene finding sub-assemblage AII and further genetic diversity within assemblage B. To better explore the local epidemiology of giardiasis and its impact on child health, additional sampling of school children with assemblage typing would be worthwhile.
作为对阿尔伯特湖沿岸五所小学学童胃肠道寄生虫进行的一项流行病学调查的一部分，通过使用属特异性18S rDNA探针的粪便样本实时PCR分析确定，贾第虫病的患病率为87.0%（n = 254）。粪便样本进一步用分类群组合特异性磷酸丙糖异构酶（TPI）Taqman®探针进行表征，并通过对贾第虫基因进行序列表征。虽然不如18S rDNA检测敏感，但TPI探针的总体患病率为52.4%，按分类群组合的患病率分别为8.3%（组合A）、35.8%（组合B）和8.3%的合并感染（A和B组合）。虽然组合B在整个样本中占主导地位，但组合A和B及其合并感染的比例因学校和儿童年龄而异；混合感染在伦加学校尤为常见（比值比[OR]=6.9[95%置信区间；2.5,19.3]），在6岁及以下儿童中也较为常见（OR = 2.7[95%置信区间；1.0,7.3]）。组合B感染与体重不足儿童有关（OR = 2.0[95%置信区间；1.0,3.9]）。通过对贾第虫基因进行序列分析，发现亚组合AII以及组合B内进一步的遗传多样性，也证实了每个组合的存在。为了更好地探索贾第虫病的当地流行病学及其对儿童健康的影响，对学童进行额外的组合分型采样将是值得的。
