School of Pharmacy, Jiangsu University, Zhenjiang, China.
School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, China.
Nat Prod Res. 2020 May;34(9):1315-1319. doi: 10.1080/14786419.2018.1560285. Epub 2019 Jan 19.
A rapid PCR technology was developed to differentiate antlers species and adulteration based on the difference in mitochondrial genome. Three specifically designed primer sets were confirmed to have high inter-species specificity and good intra-species stability. Limits of detection were estimated to be 1 ng of genomes for reindeer and 10 ng for the other species. Especially, when the mixture of antlers and reindeer or sambar was assayed, these primer sets still exhibited strong capability of differentiation but not the conventional COI barcoding. By using the newly developed approach, five batches out of fourteen commercial antler products were identified to be fake products made from reindeer antlers. It has shown its good potential to be extensively applied in the identification of counterfeits or adulterates of Cornu Chinese medicines for their pulverized and processed form, and even the traditional Chinese patent medicines composed of these species.
建立了一种快速 PCR 技术，通过线粒体基因组的差异来区分鹿茸物种和掺假。三个专门设计的引物对被证实具有高度的种间特异性和良好的种内稳定性。检测限估计为驯鹿 1ng 基因组和其他物种 10ng 基因组。特别是，当混合鹿茸和驯鹿或马鹿进行检测时，这些引物对仍然表现出很强的区分能力，但 COI 条形码不行。利用新开发的方法，在 14 批商业鹿茸产品中，有 5 批被鉴定为假冒产品，是用驯鹿鹿茸制成的。它显示出了很好的潜力，可以广泛应用于鉴定这些物种的粉末状和加工形式的中药材的假冒伪劣品，甚至是由这些物种组成的传统中药。
