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利用针对特定线粒体DNA元件的多重PCR检测法同时鉴定羚羊物种。

Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in .

作者信息

Chen Yufei, Yang Yaya, Qian Yinhuan, Gblinwon Roselyn Tehzee, Jiao Zhaoqun, Chen Liqun, Lin Ling, Zheng Yang, Yang Huan, Shen Yuping

机构信息

School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, China.

Department of Pharmacy, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang, Jiangsu, China.

出版信息

Mitochondrial DNA B Resour. 2019 Sep 25;4(2):3222-3226. doi: 10.1080/23802359.2019.1666667.

DOI:10.1080/23802359.2019.1666667
PMID:33365929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7707849/
Abstract

A multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in . Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes. When a mixture of antelope hornand fake species was assayed, it exhibited powerful differentiation capability. 5 out of 12 batches of commercialproducts were identified to be counterfeited or adulterated with Linnaeus and/or Linnaeus. It is readily applicable in routine analysis for identification of sham or adulterants of .

摘要

开发了一种多重PCR检测方法，用于同时区分四种羚羊物种并鉴定其中的掺假情况。设计了四组具有高种间特异性和种内稳定性的新型引物。检测限估计为10纳克基因组。当检测羚羊角与假冒物种的混合物时，它表现出强大的区分能力。12批商业产品中有5批被鉴定为假冒或掺有原羚和/或鹅喉羚。它很容易应用于常规分析，以鉴定羚羊角的假冒品或掺假物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bbc/7707849/6c3c81a7aa38/TMDN_A_1666667_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bbc/7707849/ba589dec58c2/TMDN_A_1666667_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bbc/7707849/6c3c81a7aa38/TMDN_A_1666667_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bbc/7707849/ba589dec58c2/TMDN_A_1666667_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bbc/7707849/6c3c81a7aa38/TMDN_A_1666667_F0002_B.jpg

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