a Department of Neurosurgery , Fujian Provincial Hospital of Fujian Medical University , Fuzhou , China.
b Department of Pathology , Fujian Provincial Hospital of Fujian Medical University , Fuzhou , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):250-255. doi: 10.1080/21691401.2018.1549062.
Flotillin-2 (FLOT2) was reported as oncogene and involves in the pathogenic process of several cancers, yet the precise mechanism of FLOT2 in glioma is still limited. In this study, we demonstrated that FLOT2 expression levels were greatly upregulated in glioma tissues and cell lines, and the FLOT2 expression in glioma tissue was markedly associated with tumour stage and size. Overexpression of FLOT2 was correlated with poor prognosis of glioma patients. The functional assay revealed that silenced FLOT2 repressed the viability, migration, and invasion of glioma cells. And then, we detected the relationship between miR-449 and FLOT2. Luciferase reporter assay and Western blot results showed that miR-449 directly binding the 3'UTR sequence of FLOT2 and regulated FLOT2 expression in glioma cells. Finally, we detected the expression levels of miR-449 in glioma tissue and cell lines and found that miR-449 was significantly downregulated in glioma tissues and cell lines. In conclusion, we demonstrated that overexpression FLOT2 was associated with poor prognosis of glioma patients and involved in the progression of glioma, identifying a novel prognostic biomarker and therapeutic target for glioma progression.
flotillin-2 (FLOT2) 被报道为癌基因，参与了几种癌症的发病过程，然而，FLOT2 在神经胶质瘤中的精确机制仍有限。在本研究中，我们证明了 FLOT2 在神经胶质瘤组织和细胞系中的表达水平显著上调，FLOT2 在神经胶质瘤组织中的表达与肿瘤分期和大小明显相关。FLOT2 的过表达与神经胶质瘤患者的不良预后相关。功能分析表明，沉默 FLOT2 抑制了神经胶质瘤细胞的活力、迁移和侵袭。然后，我们检测了 miR-449 和 FLOT2 之间的关系。荧光素酶报告基因检测和 Western blot 结果表明，miR-449 可以直接结合 FLOT2 的 3'UTR 序列并调节神经胶质瘤细胞中的 FLOT2 表达。最后，我们检测了神经胶质瘤组织和细胞系中 miR-449 的表达水平，发现 miR-449 在神经胶质瘤组织和细胞系中显著下调。总之，我们证明了高表达的 FLOT2 与神经胶质瘤患者的不良预后相关，并参与了神经胶质瘤的进展，确定了一个新的预后生物标志物和神经胶质瘤进展的治疗靶点。
