Department of Orthopaedics, Characteristic Medical Center of the Chinese People's Armed Police Force , Tianjin , China.
Cranial Brain Injury Rescue Center, Tianjin Huanhu Hospital , Tianjin , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3559-3568. doi: 10.1080/21691401.2019.1640714.
Osteosarcoma is one of the most common malignancies in children and adolescents. Studies have shown that miR-34c-5p is involved in the progression of various cancers. To explore the effects of miR-34c-5p on the proliferation, migration and invasion of osteosarcoma cells and its potential mechanism. qRT-PCR was used to detect the expression levels of miR-34c-5p and FLOT2 mRNA in osteosarcoma tissues and cells. Western Blot was used to detect protein expression. MTT assay used to detect cell viability. Transwell was used to detect cell migration and invasion in each group. Dual luciferase reporter gene assay was used to detect luciferase activity. The expression of miR-34c-5pwas significantly decreased in osteosarcoma tissues and cells and the expression level of FLOT2 mRNA was significantly increased. Overexpression of miR-34c-5p and inhibition of FLOT2 inhibited the proliferation, migration and invasion of osteosarcoma cells and inhibited the expression of Cyclin D1, MMP-2 and MMP-9 proteins and promoted the expression of p21 protein. miR-34c-5p targeted to regulate the expression of FLOT2. Overexpression of FLOT2 reversed the inhibitory effect of miR-34c-5p overexpression on proliferation, migration and invasion of osteosarcoma cell lines. miR-34c-5p can inhibit the proliferation, migration and invasion of osteosarcoma cells. The mechanism may be related to targeting FLOT2, which will provide a new target for the prevention and treatment of osteosarcoma.
骨肉瘤是儿童和青少年中最常见的恶性肿瘤之一。研究表明，miR-34c-5p 参与了多种癌症的进展。为了探讨 miR-34c-5p 对骨肉瘤细胞增殖、迁移和侵袭的影响及其潜在机制。qRT-PCR 用于检测骨肉瘤组织和细胞中 miR-34c-5p 和 FLOT2 mRNA 的表达水平。Western Blot 用于检测蛋白表达。MTT 法检测细胞活力。Transwell 检测各组细胞迁移和侵袭。双荧光素酶报告基因检测用于检测荧光素酶活性。miR-34c-5p 在骨肉瘤组织和细胞中的表达明显降低，FLOT2 mRNA 的表达水平明显升高。miR-34c-5p 的过表达和 FLOT2 的抑制抑制了骨肉瘤细胞的增殖、迁移和侵袭，并抑制了 Cyclin D1、MMP-2 和 MMP-9 蛋白的表达，促进了 p21 蛋白的表达。miR-34c-5p 靶向调节 FLOT2 的表达。FLOT2 的过表达逆转了 miR-34c-5p 过表达对骨肉瘤细胞系增殖、迁移和侵袭的抑制作用。miR-34c-5p 可抑制骨肉瘤细胞的增殖、迁移和侵袭。其机制可能与靶向 FLOT2 有关，这将为骨肉瘤的防治提供新的靶点。
