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1
Evidence for an oxyanion hole in serine beta-lactamases and DD-peptidases.丝氨酸β-内酰胺酶和DD-肽酶中氧负离子洞的证据。
Biochem J. 1988 Dec 1;256(2):669-72. doi: 10.1042/bj2560669.
2
The importance of the negative charge of beta-lactam compounds in the interactions with active-site serine DD-peptidases and beta-lactamases.β-内酰胺化合物的负电荷在与活性位点丝氨酸DD-肽酶和β-内酰胺酶相互作用中的重要性。
Biochem J. 1991 Sep 15;278 ( Pt 3)(Pt 3):801-7. doi: 10.1042/bj2780801.
3
The D-methyl group in beta-lactamase evolution: evidence from the Y221G and GC1 mutants of the class C beta-lactamase of Enterobacter cloacae P99.β-内酰胺酶进化中的D-甲基基团：来自阴沟肠杆菌P99的C类β-内酰胺酶Y221G和GC1突变体的证据
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4
Kinetics and stereochemistry of hydrolysis of an N-(phenylacetyl)-α-hydroxyglycine ester catalyzed by serine β-lactamases and DD-peptidases.丝氨酸β-内酰胺酶和 DD-肽酶催化的 N-(苯乙酰基)-α-羟基甘氨酸酯的水解动力学和立体化学。
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5
Delta 2- and delta 3-cephalosporins, penicillinate and 6-unsubstituted penems. Intrinsic reactivity and interaction with beta-lactamases and D-alanyl-D-alanine-cleaving serine peptidases.δ2-和δ3-头孢菌素、青霉素酸盐及6-未取代青霉烯。内在反应活性以及与β-内酰胺酶和D-丙氨酰-D-丙氨酸裂解丝氨酸肽酶的相互作用。
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6
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7
Peptidase activity of beta-lactamases.β-内酰胺酶的肽酶活性。
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8
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The importance of the negative charge of beta-lactam compounds for the inactivation of the active-site serine DD-peptidase of Streptomyces R61.
FEBS Lett. 1987 Dec 10;225(1-2):218-22. doi: 10.1016/0014-5793(87)81161-4.
10
A "cephalosporin-like" cyclic depsipeptide: synthesis and reaction with beta-lactam-recognizing enzymes.
Bioorg Med Chem Lett. 1999 Feb 8;9(3):341-6. doi: 10.1016/s0960-894x(98)00739-2.

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beta-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin.β-内酰胺酶通过酰基酶中间体起作用。大肠杆菌RTEM酶与头孢西丁的相互作用。
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Pre-steady state beta-lactamase kinetics. Observation of a covalent intermediate during turnover of a fluorescent cephalosporin by the beta-lactamase of STaphylococcus aureus PC1.β-内酰胺酶的预稳态动力学。金黄色葡萄球菌PC1的β-内酰胺酶在荧光头孢菌素周转过程中共价中间体的观察。
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The acyl-enzyme mechanism of beta-lactamase action. The evidence for class C Beta-lactamases.β-内酰胺酶作用的酰基-酶机制。C类β-内酰胺酶的证据。
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Inactivation of Bacillus cereus beta-lactamase I by 6 beta-bromopencillanic acid: mechanism.6β-溴青霉烷酸对蜡样芽孢杆菌β-内酰胺酶I的失活作用：作用机制
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beta-Lactamase-catalyzed hydrolysis of acyclic depsipeptides and acyl transfer to specific amino acid acceptors.β-内酰胺酶催化的无环缩肽水解及酰基转移至特定氨基酸受体。
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The active site of the P99 beta-lactamase from Enterobacter cloacae.阴沟肠杆菌P99β-内酰胺酶的活性位点。
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Chymotrypsin-catalyzed phenyl ester hydrolysis. Evidence for electrophilic assistance on carbonyl oxygen.胰凝乳蛋白酶催化的苯基酯水解。羰基氧上亲电辅助作用的证据。
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Structure of crystalline alpha-chymotrypsin. IV. The structure of indoleacryloyl-alpha-chyotrypsin and its relevance to the hydrolytic mechanism of the enzyme.结晶α-胰凝乳蛋白酶的结构。IV. 吲哚丙烯酰-α-胰凝乳蛋白酶的结构及其与该酶水解机制的相关性。
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丝氨酸β-内酰胺酶和DD-肽酶中氧负离子洞的证据。

Evidence for an oxyanion hole in serine beta-lactamases and DD-peptidases.

作者信息

Murphy B P, Pratt R F

机构信息

Department of Chemistry, Wesleyan University, Middletown, CT 06457.

出版信息

Biochem J. 1988 Dec 1;256(2):669-72. doi: 10.1042/bj2560669.

DOI:10.1042/bj2560669
PMID:3066349
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1135462/
Abstract

A thionocephalosporin is shown to be a much poorer substrate of representative serine beta-lactamases of class A (RTEM-2) and class C (Enterobacter cloacae P99) and a much poorer inhibitor of the Streptomyces R61 DD-peptidase than is the analogous oxo beta-lactam. These results provide kinetic evidence for the existence of a catalytic oxyanion hole in these enzymes.

摘要

与类似的氧代β-内酰胺相比，硫代头孢菌素是A类（RTEM-2）和C类（阴沟肠杆菌P99）代表性丝氨酸β-内酰胺酶的更差底物，也是链霉菌R61 D-肽酶的更差抑制剂。这些结果为这些酶中催化性氧阴离子洞的存在提供了动力学证据。