基于 SPIONs 的多功能两性纳米粒子的制备及其作为靶向药物传递系统的研究。

Preparation of multifunctional Janus nanoparticles on the basis of SPIONs as targeted drug delivery system.

机构信息

Polymer Laboratory, School of Chemistry, College of Science, University of Tehran, PO Box 14155 6455, Tehran, Iran.

出版信息

Int J Pharm. 2019 Mar 25;559:1-12. doi: 10.1016/j.ijpharm.2019.01.020. Epub 2019 Jan 18.

DOI:10.1016/j.ijpharm.2019.01.020

Abstract

Passing the Blood-Brain-Barrier (BBB) is a challenging aspect in nanomedicine. Utilizing surfactant particles is reported to be a potent strategy for easier BBB penetration. On the other hand, loading different functional molecules on a single particle is therapeutically and economically beneficial. In this study, multifunctional amphiphilic Janus nanoparticles have been prepared on the basis of superparamagnetic iron oxide nanoparticles. This Janus platform is armed with folic acid targeting agent and Doxorubicin (DOX) drug that have been conjugated on different sides of the nanoparticles. DOX has been conjugated via imine bond that makes these particles pH sensitive. Chemo-physical characters, in-vitro drug release pattern and toxicity of nanoparticles on rat C6 glioma cell line were studied that confirmed the preparation and pH-dependent behavior of nanoparticles. Microscopy observations showed the Janus morphology of nanoparticles and their cell penetration behavior. Prepared Janus nanoparticle can be utilized as a multifunctional nanomedicine platform for brain cancer treatment.

摘要

通过血脑屏障（BBB）是纳米医学中的一个挑战。据报道，利用表面活性剂颗粒是一种更有效的穿透 BBB 的策略。另一方面，在单个颗粒上加载不同的功能分子在治疗和经济上都是有益的。在这项研究中，基于超顺磁性氧化铁纳米颗粒制备了多功能两亲性 Janus 纳米粒子。这个 Janus 平台配备了叶酸靶向剂和阿霉素（DOX）药物，它们分别连接在纳米粒子的两侧。DOX 通过亚胺键连接，使这些颗粒具有 pH 敏感性。研究了纳米粒子的物理化学性质、体外药物释放模式和对大鼠 C6 神经胶质瘤细胞系的毒性，证实了纳米粒子的制备和 pH 依赖性行为。显微镜观察显示了纳米粒子的 Janus 形态及其细胞穿透行为。制备的 Janus 纳米粒子可用作治疗脑癌的多功能纳米医学平台。