Institute of Environmental Science and Research Limited, Private Bag 92021, Auckland, 1142 New Zealand.
Institute of Environmental Science and Research Limited, Private Bag 92021, Auckland, 1142 New Zealand.
Forensic Sci Int Genet. 2019 May;40:1-8. doi: 10.1016/j.fsigen.2019.01.006. Epub 2019 Jan 15.
An intra and inter-laboratory study using the probabilistic genotyping (PG) software STRmix™ is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded. For Sample 1 (low template, in the order of 200 rfu for major contributors) five participants described the comparison as inconclusive with respect to the POI or excluded him. Where LRs were assigned, the point estimates ranging from 2 × 10 to 8 × 10. For Sample 2 (in the order of 2000 rfu for major contributors), LRs ranged from 2 × 10 to 2 × 10. Where LRs were calculated, the differences between participants can be attributed to (from largest to smallest impact): This study demonstrates a high level of repeatability and reproducibility among the participants. For those results that differed from the mode, the differences in LR were almost always minor or conservative.
本文报告了使用概率基因分型（PG）软件 STRmix™进行的实验室内部和实验室间研究。从 PROVEDIt 集中选择了两个复杂混合物进行分析，这些混合物是在 Applied Biosystems™ 3500 系列基因分析仪上进行分析的。有 174 名参与者做出了回应。对于样本 1（低模板，主要贡献者的数量约为 200 rfu），有五名参与者表示，对于 POI 的比较不确定或排除了他。在分配 LR 时，点估计值范围从 2×10 到 8×10。对于样本 2（主要贡献者的数量约为 2000 rfu），LR 范围从 2×10 到 2×10。在计算 LR 的地方，参与者之间的差异可以归因于（从最大到最小的影响）：这项研究表明，参与者之间具有很高的可重复性和再现性。对于与模式不同的结果，LR 的差异几乎总是较小或保守的。
