转录组分析为三种紫杉属植物中几个紫杉醇生物合成相关基因的表达模式和序列相似性提供了深入了解。

Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species.

机构信息

College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.

Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310036, China.

出版信息

BMC Plant Biol. 2019 Jan 21;19(1):33. doi: 10.1186/s12870-019-1645-x.

BACKGROUND

Taxol is an efficient anticancer drug; however, the accumulation of taxoids can vary hugely among Taxus species. The mechanism underlying differential accumulation of taxoids is largely unknown. Thus, comparative analysis of the transcriptomes in three Taxus species, including T. media, T. mairei and T. cuspidata, was performed.

RESULTS

KEGG enrichment analysis revealed that the diterpenoid biosynthesis and cytochrome P450 pathways were significantly enriched in different comparisons. Differential expressions of these taxol biosynthesis related genes might be a potential explanation for the interspecific differential accumulation of taxol and its derivatives. Besides, the sequences of several MEP pathway-associated genes, such as DXS, DXR, MCT, CMK, MDS, HDS, HDR, IPPI, and GGPPS, were re-assembled based on independent transcriptomes from the three Taxus species. Phylogenetic analysis of these MEP pathway-associated enzymes also showed a high sequence similarity between T. media and T. cuspidata. Moreover, 48 JA-related transcription factor (TF) genes, including 10 MYBs, 5 ERFs, 4 RAPs, 3 VTCs, and 26 other TFs, were analyzed. Differential expression of these JA-related TF genes suggested distinct responses to exogenous JA applications in the three Taxus species.

CONCLUSIONS

Our results provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species. The data give us an opportunity to reveal the mechanism underlying the variations in the taxoid contents and to select the highest-yielding Taxus species.

背景

紫杉醇是一种有效的抗癌药物；然而，紫杉属物种之间的紫杉烷类化合物的积累量差异很大。紫杉烷类化合物积累差异的机制在很大程度上尚不清楚。因此，对包括密叶红豆杉、南方红豆杉和中国红豆杉在内的三种红豆杉属物种的转录组进行了比较分析。

结果

KEGG 富集分析显示，二萜生物合成和细胞色素 P450 途径在不同比较中显著富集。这些紫杉醇生物合成相关基因的差异表达可能是紫杉醇及其衍生物种间差异积累的潜在解释。此外，基于三种红豆杉属物种的独立转录组，重新组装了几个 MEP 途径相关基因的序列，如 DXS、DXR、MCT、CMK、MDS、HDS、HDR、IPPI 和 GGPPS。这些 MEP 途径相关酶的系统发育分析也显示密叶红豆杉和中国红豆杉之间具有很高的序列相似性。此外，分析了 48 个 JA 相关转录因子（TF）基因，包括 10 个 MYB、5 个 ERF、4 个 RAP、3 个 VTC 和 26 个其他 TF。这些 JA 相关 TF 基因的差异表达表明，三种红豆杉属物种对外源 JA 应用有不同的反应。