College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China.
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310036, China.
Plant Cell Physiol. 2017 Nov 1;58(11):1878-1890. doi: 10.1093/pcp/pcx128.
Taxus species are well-known for paclitaxel, which exhibits antitumor activities and is used for treating various cancers. Although most Taxus species are widespread in many areas, few studies have characterized the variation in metabolism among different Taxus species. Using an integrated approach involving 'tandem mass tag' labeling and liquid chromatography-tandem mass spectrometry (HPLC-MS), proteomes of T. media and T. mairei were investigated and 4078 proteins were quantified. Screening and classification of differentially expressed proteins revealed many metabolism-associated proteins. In detail, four enzymes involved in the flavonoid biosynthesis pathway were predominantly expressed in T. mairei. Four enzymes associated with supplying precursors for paclitaxel biosynthesis and three cytochrome P450 taxoid oxygenases were preferentially expressed in T. media compared with T. mairei. Furthermore, variations in taxoid contents between T. media and T. mairei were determined using HPLC-MS analysis. Variations in flavonoid contents between T. media and T. mairei were determined by HPLC analysis. A number of differentially expressed proteins may provide an explanation for the variation in metabolisms of different Taxus species.
红豆杉属植物以紫杉醇而闻名，紫杉醇具有抗肿瘤活性，用于治疗各种癌症。尽管大多数红豆杉属植物广泛分布于许多地区，但很少有研究描述不同红豆杉属植物之间代谢的变化。本研究采用“串联质量标签”标记和液相色谱-串联质谱（HPLC-MS）的综合方法，对密叶红豆杉和南方红豆杉的蛋白质组进行了研究，共定量了 4078 种蛋白质。通过筛选和分类差异表达蛋白，发现了许多与代谢相关的蛋白。具体而言，黄酮类生物合成途径中的四个酶在南方红豆杉中表达丰富。与紫杉醇生物合成前体供应相关的四个酶和三个细胞色素 P450 紫杉烷加氧酶在密叶红豆杉中比在南方红豆杉中优先表达。此外，还通过 HPLC-MS 分析确定了密叶红豆杉和南方红豆杉之间的紫杉烷含量变化。通过 HPLC 分析确定了密叶红豆杉和南方红豆杉之间的类黄酮含量变化。一些差异表达蛋白可能为不同红豆杉属植物代谢的变化提供了解释。
