Echeverria P, Hanchalay S, Taylor D N
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Diagn Microbiol Infect Dis. 1988 Jun;10(2):75-80. doi: 10.1016/0732-8893(88)90043-0.
The immunological response to plasmid-encoded antigens of virulent Shigella was determined in Thai children less than 4 yr of age and in Thai adults by immunoblot analysis and ELISA. Forty-two percent (8/19) of Thai children and 4% (1/22) of Thai adults with shigellosis developed a greater than or equal to 4-fold rise in IgG antibody titer to water-extracted antigens of Shigella flexneri M90T by ELISA (p = 0.006). Two children and one lactating mother with shigellosis developed a 4-fold rise in serum IgA antibody titers to water-extracted antigens of M90T. The results of the ELISA were confirmed by immunoblot analysis in all of the 41 paired sera examined. Five patients developed IgA, and four developed IgM, antibodies as detected by immunoblot analysis, that were not detected by ELISA. The reciprocal log2 geometric mean titers of antibodies to plasmid-encoded antigens in acute sera was higher in Thai adults than Thai children: IgG 7,265 versus 1,659; IgM 879 versus 480; and IgA 662 versus 60 (p less than 0.001). Thai adults had high titers of antibodies to plasmid-encoded antigens in their acute sera, but were susceptible to Shigella infections, although they were historically less susceptible than Thai children.
通过免疫印迹分析和酶联免疫吸附测定（ELISA），确定了泰国4岁以下儿童和泰国成年人对致病性志贺氏菌质粒编码抗原的免疫反应。在患有志贺氏菌病的泰国儿童中，42%（8/19）对福氏志贺氏菌M90T水提取物抗原的IgG抗体滴度升高了4倍或更高，而在患有志贺氏菌病的泰国成年人中这一比例为4%（1/22）（p = 0.006）。两名患有志贺氏菌病的儿童和一名哺乳期母亲对M90T水提取物抗原的血清IgA抗体滴度升高了4倍。ELISA的结果在所有检测的41份配对血清中均通过免疫印迹分析得到了证实。免疫印迹分析检测到5名患者产生了IgA抗体，4名患者产生了IgM抗体，而ELISA未检测到这些抗体。急性血清中针对质粒编码抗原的抗体的倒数log2几何平均滴度在泰国成年人中高于泰国儿童：IgG分别为7265和1659；IgM分别为879和480；IgA分别为662和60（p < 0.001）。泰国成年人急性血清中针对质粒编码抗原的抗体滴度较高，但尽管他们历来比泰国儿童不易感，仍易感染志贺氏菌。
