Lakshmikanth Tadepally, Brodin Petter
Science for Life Laboratory, Division of Clinical Paediatrics, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
Department of Newborn Medicine, Karolinska University Hospital, Stockholm, Sweden.
Methods Mol Biol. 2019;1913:33-48. doi: 10.1007/978-1-4939-8979-9_3.
As therapies involving the modulation, stimulation, and deliberate excitation of the immune system are becoming routine, better methods for monitoring immune responses in human patients are needed. Mass cytometry allows for detailed profiling of all immune cell populations and their functional responses using a simple blood sample. When combined with appropriate computational analyses, the resolution for distinguishing desired responses from unproductive or even adverse reactions to immunotherapeutic interventions increases. Here we describe a core experimental and computational framework for global, systems-level immune monitoring by mass cytometry.
随着涉及免疫系统调节、刺激和有意激发的疗法日益常规化，需要更好的方法来监测人类患者的免疫反应。质谱流式细胞术能够使用简单的血液样本对所有免疫细胞群体及其功能反应进行详细分析。当与适当的计算分析相结合时，区分免疫治疗干预的期望反应与无效甚至不良反应的分辨率会提高。在此，我们描述了一个通过质谱流式细胞术进行全局、系统水平免疫监测的核心实验和计算框架。
