Punt P J, Dingemanse M A, Jacobs-Meijsing B J, Pouwels P H, van den Hondel C A
TNO Medical Biological Laboratory, Rijswijk, The Netherlands.
Gene. 1988 Sep 15;69(1):49-57. doi: 10.1016/0378-1119(88)90377-0.
The isolation and characterization of the highly expressed glyceraldehyde-3-phosphate dehydrogenase (GPD)-coding gene (gpdA) of Aspergillus nidulans is described. The gene was isolated from an A. nidulans lambda gene library with a Saccharomyces cerevisiae GPD-coding gene as a probe. Unlike many other eukaryotes, A. nidulans contains only one GPD-coding gene. At the amino acid level, homology with other GPD enzymes is extensive. The A. nidulans gene contains seven introns, one of which is positioned in the 5'-untranslated part of the gene. The major transcription start point is found at 172 bp upstream from the start codon. Polyadenylation occurs at several sites about 200 bp downstream from the stop codon. Comparison of 5' and 3' flanking sequences with flanking sequences of other highly expressed (glycolytic) genes shows several regions of similar sequence.
本文描述了构巢曲霉中高表达的3-磷酸甘油醛脱氢酶（GPD）编码基因（gpdA）的分离与鉴定。该基因是用酿酒酵母GPD编码基因作为探针，从构巢曲霉λ基因文库中分离得到的。与许多其他真核生物不同，构巢曲霉仅含有一个GPD编码基因。在氨基酸水平上，它与其他GPD酶具有广泛的同源性。构巢曲霉基因含有7个内含子，其中一个位于基因的5'非翻译区。主要转录起始点位于起始密码子上游172 bp处。聚腺苷酸化发生在终止密码子下游约200 bp的几个位点。将5'和3'侧翼序列与其他高表达（糖酵解）基因的侧翼序列进行比较，发现了几个序列相似的区域。
