Okuda Y, Taomoto K, Saya H, Ijichi A, Kokunai T, Tamaki N, Matsumoto S
Department of Neurosurgery, Kobe University School of Medicine, Japan.
J Neurooncol. 1988 Sep;6(2):193-8. doi: 10.1007/BF02327396.
Immunohistochemical and immunocytochemical studies were performed on a specimen obtained at operation from a 4 year-old female with intracranially spread pineal tumor in the right temporal lobe. Microscopically, it was a highly cellular tumor, and the cells contained chromatin-rich round or oval nuclei and scanty cytoplasms. There were numerous mitotic figures. Rosettes were not detected. Both immunohistochemical staining of paraffin-embedded sections and immunocytochemical staining of short-term cultured cells disclosed a subgroups of cell positive for NSE and 68K-NF (neurofilament). No cells were positive for GFAP, S-100 protein or HCG. From these findings, the tumor was thought to be pineoblastoma, which is an undifferentiated tumor that rarely shows divergent differentiating potential. This is the first report of pineoblastoma with the differentiation only toward the neuronal line confirmed by the analysis of immunohistochemistry and immunocytochemistry.
对一名4岁患有右颞叶颅内播散性松果体瘤的女性患者手术获取的标本进行了免疫组织化学和免疫细胞化学研究。显微镜下，这是一种细胞高度丰富的肿瘤，细胞含有富含染色质的圆形或椭圆形细胞核以及少量细胞质。有大量有丝分裂象。未检测到菊形团。石蜡包埋切片的免疫组织化学染色和短期培养细胞的免疫细胞化学染色均显示出一组对NSE和68K-NF（神经丝）呈阳性的细胞亚群。没有细胞对GFAP、S-100蛋白或HCG呈阳性。根据这些发现，该肿瘤被认为是松果体母细胞瘤，这是一种未分化肿瘤，很少表现出不同的分化潜能。这是首例通过免疫组织化学和免疫细胞化学分析证实仅向神经系分化的松果体母细胞瘤报告。
