Laboratory for Developmental Neurobiology, Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, Japan; Laboratory of Single-Molecule Cell Biology, Kyoto University Graduate School of Biostudies, Kyoto, Japan.
Laboratory for Developmental Neurobiology, Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, Japan.
Cell Calcium. 2019 Mar;78:76-80. doi: 10.1016/j.ceca.2019.01.001. Epub 2019 Jan 4.
Extracellular stimuli evoke a robust increase in the concentration of intracellular Ca ([Ca]) throughout the cell to trigger various cellular responses, such as gene expression and apoptosis. This robust expansion of [Ca] is called Ca propagation. To date, it is thought that intracellular second messengers, such as inositol 1,4,5-trisphosphate (IP) and intracellular Ca, and clusters of IP receptors (IPRs) regulate Ca propagation. However, little is known about how the elevation in the [Ca] spreads throughout the cell, especially in non-polar cell, including HeLa cell. In this study, we developed a novel multi lines analysis tool. This tool revealed that the velocity of Ca propagation was inconstant throughout cell and local concentration of intracellular Ca did not contribute to the velocity of Ca propagation. Our results suggest that intracellular Ca propagation is not merely the result of diffusion of intracellular Ca, and that, on the contrary, intracellular Ca propagation seems to be regulated by more complicated processes.
细胞外刺激会引发细胞内钙离子浓度([Ca])的剧烈增加，从而触发各种细胞反应，如基因表达和细胞凋亡。这种[Ca]的剧烈扩张被称为钙波传播。迄今为止，人们认为细胞内的第二信使，如肌醇 1,4,5-三磷酸（IP）和细胞内 Ca，以及 IP 受体（IPR）簇，调节钙波传播。然而，人们对[Ca]如何在细胞内传播，特别是在非极性细胞（包括 HeLa 细胞）中的传播知之甚少。在这项研究中，我们开发了一种新的多线分析工具。该工具表明，钙波传播的速度在整个细胞中是不一致的，细胞内 Ca 的局部浓度对钙波传播的速度没有贡献。我们的结果表明，细胞内 Ca 波传播不仅仅是细胞内 Ca 扩散的结果，相反，细胞内 Ca 波传播似乎受到更复杂的过程的调节。
