Key Laboratory of Crop Ecophysiology and Farming System in Southwest China of Ministry of Agriculture, Sichuan Agricultural University, Chengdu, China.
Institute for New Rural Development, Sichuan Agricultural University, Ya'an, China.
Sci Rep. 2019 Jan 22;9(1):313. doi: 10.1038/s41598-018-36371-7.
Increase in grain nitrogen concentration (GNC), which is directly affected by nitrogen (N) application, can help overcome the issues of malnutrition. Here, the effects of urea type (polyaspartic acid (PASP) urea and conventional urea) and N management method (two splits and four splits) on GNC and N concentration of head rice were investigated in field experiments conducted in Sichuan, China, in 2014 and 2015. N concentration of grain and head rice were significantly (P < 0.05) increased by N redistribution from the leaf lamina, activities of glutamine synthetase (GS), and glutamate synthase (GOGAT) at the heading stage, and N concentration and GOGAT activity in the leaf lamina at the maturity stage. Compared to conventional urea, PASP-urea significantly improved N concentration of grain and head rice by improving the activities of GS and GOGAT, thereby increasing N distribution in the leaf lamina. The four splits method, unlike the two splits method, enhanced N concentration and activities of key N metabolism enzymes of leaf lamina, leading to increased GNC and N concentration in head rice too. Overall, four splits is a feasible method for using PASP-urea and improving GNC.
增加谷物氮浓度(GNC),这直接受到氮(N)应用的影响,有助于克服营养不良问题。在这里,研究了尿素类型(多聚天冬氨酸(PASP)尿素和传统尿素)和 N 管理方法(两次分施和四次分施)对 2014 年和 2015 年在中国四川进行的田间试验中 GNC 和糙米 N 浓度的影响。通过在抽穗期从叶片中重新分配氮、谷氨酰胺合成酶(GS)和谷氨酸合酶(GOGAT)的活性,以及在成熟期叶片中氮浓度和 GOGAT 活性,显著提高了籽粒和糙米的氮浓度(P<0.05)。与传统尿素相比,PASP-urea 通过提高 GS 和 GOGAT 的活性,显著改善了籽粒和糙米的氮浓度,从而增加了叶片中的氮分配。与两次分施法不同,四次分施法增强了叶片中氮代谢关键酶的活性,从而提高了 GNC 和糙米的氮浓度。总的来说,四次分施是使用 PASP-urea 和提高 GNC 的可行方法。