Kim Wong-Kyong, Moon Ja-Young, Cho Jeong-Sang, Ochirkhuyag Enkhsaikhan, Akanda Md Rashedunnabi, Park Byung-Yong, Hur Jin
Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Gobong-ro 79, Iksan, Jeollabuk-do, Republic of Korea (Kim, Moon, Cho, Ochirkhuyag, Hur); Veterinary Histology, College of Veterinary Medicine, Chonbuk National University, Gobong-ro 79, Iksan, Jeollabuk-do, Republic of Korea (Akanda, Park).
Can J Vet Res. 2019 Jan;83(1):68-74.
The efficacy of GI24-lysed cells as a vaccine candidate against brucellosis in goats was evaluated on 2 groups of Korean black goats. Group A goats were immunized subcutaneously (SC) with sterile phosphate-buffered saline, whereas group B goats were immunized SC with approximately 3 × 10 lysed cells. Subcutaneous immunization with the lysed cells did not cause any negative impact on the overall clinical status, such as behavior and appetite, throughout the study period. The enzyme-linked immunosorbent assay (ELISA) optical densities values for lipopolysaccharide in serum were considerably higher in group B than those in group A. Also, the levels of the cytokines interleukin 4 (IL-4), tumor necrosis factor-alpha (TNF-α), and interferon gamma (IFN-γ) were significantly elevated in group B compared with those in group A. Following intraconjunctival challenge with strain 544, the severity of brucellosis in terms of infection index and colonization of in tissues was significantly lower in group B than in group A. The present study concluded that 3 of 5 goats immunized with GI24-lysed bacteria were completely protected against challenge. Future investigations are required to improve the protective efficacy offered by lysed cells for practical applications in small ruminants.
在两组韩国黑山羊上评估了GI24裂解细胞作为山羊布鲁氏菌病候选疫苗的效力。A组山羊皮下注射（SC）无菌磷酸盐缓冲盐水，而B组山羊皮下注射约3×10个裂解细胞。在整个研究期间，用裂解细胞进行皮下免疫对整体临床状况（如行为和食欲）没有造成任何负面影响。B组血清中脂多糖的酶联免疫吸附测定（ELISA）光密度值明显高于A组。此外，与A组相比，B组细胞因子白细胞介素4（IL-4）、肿瘤坏死因子-α（TNF-α）和干扰素γ（IFN-γ）的水平显著升高。在用544菌株进行结膜内攻毒后，B组布鲁氏菌病在感染指数和组织定植方面的严重程度明显低于A组。本研究得出结论，用GI24裂解细菌免疫的5只山羊中有3只完全受到保护，免受攻毒。未来需要进行研究，以提高裂解细胞在小型反刍动物实际应用中的保护效力。
