Pedersen Kerri, Quance Christine R, Robbe-Austerman Suelee, Piaggio Antoinette J, Bevins Sarah N, Goldstein Samuel M, Gaston Wesson D, DeLiberto Thomas J
1 National Wildlife Research Center, Animal and Plant Health Inspection Service, Wildlife Services, US Department of Agriculture, 4101 LaPorte Avenue, Fort Collins, Colorado 80521, USA.
J Wildl Dis. 2014 Apr;50(2):171-9. doi: 10.7589/2013-09-235. Epub 2014 Jan 31.
Serologic tests currently available for brucellosis diagnosis detect antibodies to Brucella but do not distinguish between species of Brucella. Although Brucella suis is known to circulate within various feral swine (Sus scrofa) populations, our objective was to determine the primary species of Brucella circulating in feral swine populations in areas of the US with high brucellosis prevalence. We cultured lymph nodes from 183 feral swine. We identified 22 isolates from 21 animals, and all isolates were genotyped as B. suis. Most isolates were B. suis biovar 1, with the exception of two genetically distinct isolates from one feral swine in Hawaii, which were identified as B. suis biovar 3. Serum from each feral swine was also tested by the fluorescence polarization assay when possible, but only 52% (95% CL = 29.8-74.3) of culture-positive animals were antibody positive. Our results indicate that brucellosis infections in feral swine within the US are typically caused by B. suis. However, improved serologic tests are needed to more accurately determine exposure to Brucella spp. and to monitor disease trends in feral swine populations.
目前可用于布鲁氏菌病诊断的血清学检测可检测出针对布鲁氏菌的抗体，但无法区分布鲁氏菌的种类。虽然已知猪布鲁氏菌在各种野猪（Sus scrofa）种群中传播，但我们的目标是确定在美国布鲁氏菌病患病率高的地区，野猪种群中传播的布鲁氏菌的主要种类。我们培养了183头野猪的淋巴结。我们从21只动物中鉴定出22株分离株，所有分离株均被基因分型为猪布鲁氏菌。大多数分离株为猪布鲁氏菌生物变种1，但来自夏威夷一头野猪的两株基因不同的分离株除外，它们被鉴定为猪布鲁氏菌生物变种3。在可能的情况下，还对每头野猪的血清进行了荧光偏振试验检测，但只有52%（95%可信区间=29.8-74.3）的培养阳性动物抗体呈阳性。我们的结果表明，美国野猪中的布鲁氏菌病感染通常由猪布鲁氏菌引起。然而，需要改进血清学检测，以便更准确地确定接触布鲁氏菌属的情况，并监测野猪种群中的疾病趋势。
