Bons N, Bouille C, Tonon M C, Guillaume V
Laboratoire de Biologie Evolutive des Vertébrés, Ecole Pratique des Hautes Etudes, Montpellier, France.
Peptides. 1988 Jul-Aug;9(4):697-707. doi: 10.1016/0196-9781(88)90109-x.
The distribution of corticotropin-releasing factor (CRF) immunoreactivity was demonstrated by immunocytochemistry in intact and colchicine-treated pigeons. Colchicine injections were administered at different times related to the circadian activity of the CRF-adrenocorticotropin (ACTH)-corticosterone axis. Three CRF antisera were used, two directed against synthetic rat CRF and one directed against synthetic ovine CRF. No fundamental differences appeared in the pigeon brain with respect to the specific CRF antiserum used. The most effective colchicine injection times corresponded to hypersecretion in the corticotropic axis. CRF-immunopositive neurons were scattered throughout the pigeon brain. In addition to the paraventricular hypothalamic system, which is involved in adenohypophysial ACTH regulation, several other hypothalamic and extrahypothalamic areas showed CRF neurons. The distribution suggests that CRF may also act as a modulator and a neurotransmitter. Two hypothalamic paraventricular nucleus-median eminence CRF pathways are described here. Moreover, CRF-immunopositive reactions were observed in specific areas of cerebral ventricle walls, suggesting that CRF may be released into the cerebral fluid.
通过免疫细胞化学方法，在完整的和经秋水仙碱处理的鸽子体内证实了促肾上腺皮质激素释放因子（CRF）免疫反应性的分布。秋水仙碱注射在与CRF-促肾上腺皮质激素（ACTH）-皮质酮轴的昼夜活动相关的不同时间进行。使用了三种CRF抗血清，两种针对合成大鼠CRF，一种针对合成绵羊CRF。就所使用的特定CRF抗血清而言，鸽子大脑中未出现根本差异。最有效的秋水仙碱注射时间与促肾上腺皮质激素轴的分泌过多相对应。CRF免疫阳性神经元散布于整个鸽子大脑。除了参与腺垂体ACTH调节的下丘脑室旁系统外，其他几个下丘脑和下丘脑外区域也显示有CRF神经元。这种分布表明CRF可能还充当调节剂和神经递质。本文描述了两条下丘脑室旁核-正中隆起CRF通路。此外，在脑室壁的特定区域观察到CRF免疫阳性反应，表明CRF可能释放到脑脊液中。
