[鸽子脑中促肾上腺皮质激素释放因子神经元的免疫荧光定位]

[Immunofluorescence localization of corticoliberin neurons in the brain of pigeons].

作者信息

Bons N, Bouillé C, Vaudry H, Guillaume V

出版信息

C R Acad Sci III. 1985;300(2):49-52.

Abstract

With immunofluorescence techniques using one anti-rat or two different anti-ovine CRF, the localization of corticotropin-releasing factor (CRF) producing neurons was characterized in frozen sections of pigeon brain. Colchicine was administered intraventricularly at various day hours. The CRF neurons were localized in the telencephalon: lobus parolfactorius, nucleus (n.) accumbens, anterior commissure; in the diencephalon: n. dorso-medialis and lateralis thalami and in different structures of the hypothalamus: n. praeopticus periventricularis and medialis, paraventricularis, supraopticus medialis, lateralis, ectomamillaris and in the stratum cellulare externum. Concerning the hypothalamic localizations, results are discussed in the light of physiological studies on corticotropic regulations in pigeons. Additional populations of CRF neurons were also located in various brainstem areas substantia grisea centralis, locus caeruleus, n. tegmenti dorsalis, sensorius principalis nervi trigemini, vestibularis latetalis, solitarius, nervi hypoglossi, in the dorsal area of the n. pontis lateralis and in the n. paramedianus paragiganto--cellularis, raphes, nervi facialis, subcaeruleus and the area ventralis. These particular localizations may lead to the assumption that CRF might be involved in nervous regulations other than those related to the corticotropic function.

摘要

运用免疫荧光技术，使用一种抗大鼠或两种不同的抗羊促肾上腺皮质激素释放因子（CRF），在鸽脑冰冻切片中对产生促肾上腺皮质激素释放因子（CRF）的神经元进行定位。在不同的日间时段向脑室内注射秋水仙碱。CRF神经元定位于端脑：嗅旁叶、伏隔核、前连合；间脑：丘脑背内侧核和外侧核，以及下丘脑的不同结构：室周视前核和内侧视前核、室旁核、视上内侧核、视上外侧核、乳头体外侧核，以及外侧细胞层。关于下丘脑的定位，根据对鸽促肾上腺皮质激素调节的生理学研究对结果进行了讨论。另外，在脑干的各个区域也发现了CRF神经元群体，如中央灰质、蓝斑、被盖背核、三叉神经主感觉核、外侧前庭核、孤束核、舌下神经核、脑桥外侧核背侧区域、旁巨细胞旁正中核、中缝核、面神经核、蓝斑下核和腹侧区域。这些特殊的定位可能使人推测CRF可能参与了除与促肾上腺皮质激素功能相关之外的其他神经调节。