Sarmento Dmitry José de Santana, Palmieri Michelle, Galvão Gustavo Souza, Tozetto-Mendoza Tânia Regina, Canto Cynthia Motta do, Pierrotti Ligia Camera, David-Neto Elias, Agena Fabiana, Gallottini Marina, Pannuti Claudio Sergio, Fink Maria Cristina Domingues, Braz-Silva Paulo Henrique
University of São Paulo, School of Dentistry, Stomatology Department, São Paulo, São Paulo, Brazil.
University of São Paulo, Institute of Tropical Medicine of São Paulo, Laboratory of Virology, São Paulo, São Paulo, Brazil.
J Appl Oral Sci. 2019 Jan 14;27:e20180435. doi: 10.1590/1678-7757-2018-0435.
This study aimed to verify the presence of polyomavirus BK (BKPyV) in the saliva of kidney transplant recipients and to correlate it with blood viremia.
We have conducted a cross-sectional study with a sample involving 126 renal transplant recipients. 126 samples of saliva and 52 samples of blood were collected from these patients. Detection and quantification of BKPyV were performed using a real-time PCR. To compare the presence of BKPyV in blood and saliva, the binomial proportion test was used. To verify associations between salivary shedding BKPyV and post-transplant periods (in months), the Mann-Whitney test was used. Spearman's correlation was used to correlate the viral load in the saliva with blood of kidney transplant recipients.
The mean age of the study group was 51.11±12.45 years old, and 69 participants (54.8%) were female, with a mean post-transplantation time of 4.80±6.04 months. BKPyV was quantified in several samples of saliva and blood, with medians of 1,108 cp/mL and 1,255 cp/mL, respectively. Only 16/52 (30.8%) participants presented BKPyV in blood, and 59/126 (46.8%) excreted the virus in saliva (p=0.004). BKPyV shedding was found in patients at a shorter post-transplantation period (3.86±5.25, p=0.100). A weak correlation was observed between viral quantification in saliva and blood (Spearman's correlation coefficient=0.193).
The results of this study suggested that, although saliva excretes more BKPyV than blood, there is no reliable correlation between salivary shedding and blood viremia, showing two independent compartments of viral replication.
本研究旨在验证肾移植受者唾液中多瘤病毒BK(BKPyV)的存在,并将其与血液病毒血症相关联。
我们对126名肾移植受者进行了一项横断面研究。从这些患者中收集了126份唾液样本和52份血液样本。使用实时PCR对BKPyV进行检测和定量。为比较血液和唾液中BKPyV的存在情况,采用二项比例检验。为验证唾液中BKPyV脱落与移植后时间(以月为单位)之间的关联,采用曼-惠特尼检验。使用斯皮尔曼相关性分析肾移植受者唾液和血液中的病毒载量。
研究组的平均年龄为51.11±12.45岁,69名参与者(54.8%)为女性,移植后平均时间为4.80±6.04个月。在几份唾液和血液样本中对BKPyV进行了定量,中位数分别为1108拷贝/毫升和1255拷贝/毫升。只有16/52(30.8%)的参与者血液中存在BKPyV,59/126(46.8%)的参与者唾液中排出该病毒(p=0.004)。在移植后较短时间的患者中发现了BKPyV脱落(3.86±5.25,p=0.100)。唾液和血液中的病毒定量之间观察到弱相关性(斯皮尔曼相关系数=0.193)。
本研究结果表明,尽管唾液中排出的BKPyV比血液中多,但唾液脱落与血液病毒血症之间没有可靠的相关性,显示出病毒复制的两个独立区域。