Suppr超能文献

肾移植受者的BK病毒唾液排出及病毒血症

BK virus salivary shedding and viremia in renal transplant recipients.

作者信息

Sarmento Dmitry José de Santana, Palmieri Michelle, Galvão Gustavo Souza, Tozetto-Mendoza Tânia Regina, Canto Cynthia Motta do, Pierrotti Ligia Camera, David-Neto Elias, Agena Fabiana, Gallottini Marina, Pannuti Claudio Sergio, Fink Maria Cristina Domingues, Braz-Silva Paulo Henrique

机构信息

University of São Paulo, School of Dentistry, Stomatology Department, São Paulo, São Paulo, Brazil.

University of São Paulo, Institute of Tropical Medicine of São Paulo, Laboratory of Virology, São Paulo, São Paulo, Brazil.

出版信息

J Appl Oral Sci. 2019 Jan 14;27:e20180435. doi: 10.1590/1678-7757-2018-0435.

Abstract

OBJECTIVES

This study aimed to verify the presence of polyomavirus BK (BKPyV) in the saliva of kidney transplant recipients and to correlate it with blood viremia.

MATERIAL AND METHODS

We have conducted a cross-sectional study with a sample involving 126 renal transplant recipients. 126 samples of saliva and 52 samples of blood were collected from these patients. Detection and quantification of BKPyV were performed using a real-time PCR. To compare the presence of BKPyV in blood and saliva, the binomial proportion test was used. To verify associations between salivary shedding BKPyV and post-transplant periods (in months), the Mann-Whitney test was used. Spearman's correlation was used to correlate the viral load in the saliva with blood of kidney transplant recipients.

RESULTS

The mean age of the study group was 51.11±12.45 years old, and 69 participants (54.8%) were female, with a mean post-transplantation time of 4.80±6.04 months. BKPyV was quantified in several samples of saliva and blood, with medians of 1,108 cp/mL and 1,255 cp/mL, respectively. Only 16/52 (30.8%) participants presented BKPyV in blood, and 59/126 (46.8%) excreted the virus in saliva (p=0.004). BKPyV shedding was found in patients at a shorter post-transplantation period (3.86±5.25, p=0.100). A weak correlation was observed between viral quantification in saliva and blood (Spearman's correlation coefficient=0.193).

CONCLUSION

The results of this study suggested that, although saliva excretes more BKPyV than blood, there is no reliable correlation between salivary shedding and blood viremia, showing two independent compartments of viral replication.

摘要

目的

本研究旨在验证肾移植受者唾液中多瘤病毒BK(BKPyV)的存在,并将其与血液病毒血症相关联。

材料与方法

我们对126名肾移植受者进行了一项横断面研究。从这些患者中收集了126份唾液样本和52份血液样本。使用实时PCR对BKPyV进行检测和定量。为比较血液和唾液中BKPyV的存在情况,采用二项比例检验。为验证唾液中BKPyV脱落与移植后时间(以月为单位)之间的关联,采用曼-惠特尼检验。使用斯皮尔曼相关性分析肾移植受者唾液和血液中的病毒载量。

结果

研究组的平均年龄为51.11±12.45岁,69名参与者(54.8%)为女性,移植后平均时间为4.80±6.04个月。在几份唾液和血液样本中对BKPyV进行了定量,中位数分别为1108拷贝/毫升和1255拷贝/毫升。只有16/52(30.8%)的参与者血液中存在BKPyV,59/126(46.8%)的参与者唾液中排出该病毒(p=0.004)。在移植后较短时间的患者中发现了BKPyV脱落(3.86±5.25,p=0.100)。唾液和血液中的病毒定量之间观察到弱相关性(斯皮尔曼相关系数=0.193)。

结论

本研究结果表明,尽管唾液中排出的BKPyV比血液中多,但唾液脱落与血液病毒血症之间没有可靠的相关性,显示出病毒复制的两个独立区域。

相似文献

1
BK virus salivary shedding and viremia in renal transplant recipients.
J Appl Oral Sci. 2019 Jan 14;27:e20180435. doi: 10.1590/1678-7757-2018-0435.
2
Prevalence of polyoma BK virus infection among living-donor renal transplant recipients.
Transpl Infect Dis. 2016 Aug;18(4):529-37. doi: 10.1111/tid.12557. Epub 2016 Jul 29.
4
Impact of donor BK polyomavirus replication on recipient infections in living donor transplantation.
Transpl Infect Dis. 2018 Aug;20(4):e12917. doi: 10.1111/tid.12917. Epub 2018 Jun 11.
6
BK and JC virus infections in healthy patients compared to kidney transplant recipients in Tunisia.
Microb Pathog. 2016 Aug;97:204-8. doi: 10.1016/j.micpath.2016.06.015. Epub 2016 Jun 16.
7
Determination of viremia cut-off for risk to develop BKPyV-associated nephropathy among kidney transplant recipients.
Transpl Infect Dis. 2018 Oct;20(5):e12969. doi: 10.1111/tid.12969. Epub 2018 Sep 3.
8
BK virus replication in renal transplant recipients: Analysis of potential risk factors may contribute in reactivation.
J Clin Virol. 2017 Nov;96:7-11. doi: 10.1016/j.jcv.2017.09.004. Epub 2017 Sep 8.
10
BK and JC polyomavirus infections in Tunisian renal transplant recipients.
J Med Virol. 2015 Oct;87(10):1788-95. doi: 10.1002/jmv.24234. Epub 2015 May 7.

引用本文的文献

1
The Emergence of Saliva as a Diagnostic and Prognostic Tool for Viral Infections.
Viruses. 2024 Nov 11;16(11):1759. doi: 10.3390/v16111759.
2
Translating genomic exploration of the family into confident human polyomavirus detection.
iScience. 2021 Dec 11;25(1):103613. doi: 10.1016/j.isci.2021.103613. eCollection 2022 Jan 21.
3
Detection of polyomavirus microRNA-5p expression in saliva shortly after kidney transplantation.
J Oral Microbiol. 2021 Mar 12;13(1):1898838. doi: 10.1080/20002297.2021.1898838.

本文引用的文献

1
Polyomavirus microRNA in saliva reveals persistent infectious status in the oral cavity.
Virus Res. 2018 Apr 2;249:1-7. doi: 10.1016/j.virusres.2018.03.002. Epub 2018 Mar 6.
2
Polyoma virus nephropathy in kidney transplantation.
World J Transplant. 2017 Dec 24;7(6):329-338. doi: 10.5500/wjt.v7.i6.329.
3
Human saliva as a diagnostic material.
Adv Med Sci. 2018 Mar;63(1):185-191. doi: 10.1016/j.advms.2017.11.002. Epub 2017 Nov 14.
4
Detection of human polyomaviruses JC and BK in liver pretransplant patients.
Oral Dis. 2017 Nov;23(8):1127-1133. doi: 10.1111/odi.12707. Epub 2017 Jul 21.
5
Polyomavirus BK and JC in individuals with chronic kidney failure, kidney transplantation, and healthy controls.
J Clin Virol. 2017 Apr;89:5-9. doi: 10.1016/j.jcv.2017.02.003. Epub 2017 Feb 6.
6
BK and JC virus infections in healthy patients compared to kidney transplant recipients in Tunisia.
Microb Pathog. 2016 Aug;97:204-8. doi: 10.1016/j.micpath.2016.06.015. Epub 2016 Jun 16.
7
BK Virus Nephropathy in Kidney Transplantation: An Approach Proposal and Update on Risk Factors, Diagnosis, and Treatment.
Transplant Proc. 2015 Jul-Aug;47(6):1777-85. doi: 10.1016/j.transproceed.2015.05.010.
9
Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: 
a systematic review.
Biochem Med (Zagreb). 2015 Jun 5;25(2):177-92. doi: 10.11613/BM.2015.018. eCollection 2015.
10
Evaluation of Infectious Complications in the First Year After Kidney Transplantation.
Transplant Proc. 2015 Jun;47(5):1429-32. doi: 10.1016/j.transproceed.2015.04.056.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验