Stomatology Department, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Laboratory of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo, São Paulo, Brazil.
Oral Dis. 2017 Nov;23(8):1127-1133. doi: 10.1111/odi.12707. Epub 2017 Jul 21.
This study aimed to identify and quantify polyomaviruses (BKPyV and JCPyV) in the saliva, mouthwash, blood and urine of liver pretransplant patients.
A case-control study was performed using a convenience sample of 21 end-stage liver disease patients (EG = experimental group) and 20 normoreactive controls (CG = control group). In total, 162 samples were collected. Detection and quantification of polyomaviruses were performed using real-time PCR method.
In the EG, 21 samples (25%) were positive for BKPyV and 10 (11.90%) for JCPyV, while in the CG, 27 samples (34.61%) were positive for BKPyV and six (7.69%) for JCPyV. With regard to the number of samples positive for BKPyV and JCPyV, there was no statistically significant difference between EG and CG (p = .52 and p = .25). In the EG, we observed a panorama similar to that of the CG regarding the presence of polyomaviruses in mouthwash, blood and urine. The greatest difference between the samples was that regarding the identification of BKPyV in saliva.
Cirrhotic patients on the liver transplant waiting list did not show higher prevalence of BKPyV and JCPyV compared to normoreactive controls.
本研究旨在鉴定和定量分析肝移植前患者唾液、漱口液、血液和尿液中的多瘤病毒（BKPyV 和 JCPyV）。
采用方便取样的方法，进行了一项病例对照研究，纳入 21 例终末期肝病患者（EG=实验组）和 20 例正常反应者（CG=对照组）。共采集 162 份样本。采用实时 PCR 法检测和定量多瘤病毒。
在 EG 中，21 份样本（25%）BKPyV 阳性，10 份样本（11.90%）JCPyV 阳性，而在 CG 中，27 份样本（34.61%）BKPyV 阳性，6 份样本（7.69%）JCPyV 阳性。关于 BKPyV 和 JCPyV 阳性样本数量，EG 和 CG 之间无统计学差异（p=.52 和 p=.25）。在 EG 中，我们观察到多瘤病毒在漱口液、血液和尿液中的存在与 CG 相似。样本之间最大的差异在于唾液中 BKPyV 的鉴定。
与正常反应者相比，肝移植等待名单上的肝硬化患者并未显示出 BKPyV 和 JCPyV 更高的流行率。
