Department of Plant Pathology, University of Stellenbosch, Matieland 7602, South Africa, and Rwanda Agriculture and Animal Resources Development Board, Kigali, Rwanda.
Department of Plant Pathology, University of Stellenbosch, Matieland 7602, South Africa.
Plant Dis. 2018 Mar;102(3):552-560. doi: 10.1094/PDIS-02-17-0282-RE. Epub 2018 Jan 9.
Banana Fusarium wilt is a major production constraint globally and a significant threat to the livelihoods of millions of people in East and Central Africa (ECA). A proper understanding of the diversity and population dynamics of the causal agent, Fusarium oxysporum f. sp. cubense (Foc), could be useful for the development of sustainable disease management strategies for the pathogen. The current study investigated the diversity of Foc in ECA using vegetative compatibility group (VCG) analysis, PCR-RFLPs of the ribosomal DNA's intergenic spacer region, as well as phylogenetic analysis of the elongation factor-1α gene. Six VCGs (0124, 0125, 0128, 01212, 01220, and 01222), which all belong to one lineage (Foc lineage VI), were widely distributed throughout the region. VCGs 0128 and 01220 are reported for the first time in Burundi, the Democratic Republic of Congo (DRC), Rwanda, Tanzania, and Uganda, while VCG 01212 is reported in the DRC and Rwanda. Isolates that did not belong to any of the known VCGs were identified as Foc lineage VI members by phylogenetic analysis and may represent novel VCGs. CAV 2734, a banana pathogen collected in Rwanda, clustered with nonpathogenic F. oxysporum isolates in lineage VIII. Results from this study will contribute significantly toward the implementation of banana Fusarium wilt disease management practices in the region, such as the restricted movement of infected planting material and the selective planting of resistant banana varieties.
香蕉枯萎病是全球主要的生产制约因素，也是东非和中非数百万人民生计的重大威胁。正确理解病原菌尖孢镰刀菌古巴专化型（Foc）的多样性和种群动态，对于制定可持续的病害管理策略具有重要意义。本研究利用营养体亲和群（VCG）分析、核糖体 DNA 间区 PCR-RFLP 分析以及延伸因子-1α基因的系统发育分析，研究了东非和中非的 Foc 多样性。六个 VCG（0124、0125、0128、01212、01220 和 01222）广泛分布在该地区，均属于一个谱系（Foc 谱系 VI）。VCG 0128 和 01220 首次在布隆迪、刚果民主共和国（DRC）、卢旺达、坦桑尼亚和乌干达报道，而 VCG 01212 则在 DRC 和卢旺达报道。通过系统发育分析鉴定出不属于任何已知 VCG 的分离物为 Foc 谱系 VI 成员，可能代表新的 VCG。在卢旺达收集的香蕉病原菌 CAV 2734 与谱系 VIII 中的非致病性 F. oxysporum 分离物聚类。本研究结果将为该地区实施香蕉枯萎病管理实践做出重大贡献，例如限制感病种植材料的传播和选择性种植抗性香蕉品种。
