Kaushal Manoj, Mahuku George, Swennen Rony
International Institute of Tropical Agriculture (IITA), Mikocheni B, Dar es Salaam-34441, Tanzania.
Bioversity International, Willem De Croylaan 42, B-3001 Leuven, Belgium.
Plants (Basel). 2020 Feb 18;9(2):263. doi: 10.3390/plants9020263.
Plants tissues are colonized by diverse communities of microorganisms called endophytes. They are key determinants of plant production and health, for example by facilitating nutrient exchanges or limiting disease development. Endophytic communities of banana plants have not been studied until very recently, and their potential role in disease development has not been explored so far. Roots from symptomatic and non-symptomatic banana plants were sampled from fields infected by f.sp. race 1. The goal was to compare the endophytic microbiota between symptomatic and non-symptomatic plants through high throughput sequencing of 16s rDNA and shotgun metagenome sequencing. The results revealed that the endophytic root microbiome in bananas is dominated by Proteobacteria and Bacteroidetes followed to a lesser extent by Actinobacteria. The development of disease greatly impacted the endophytic microbial communities. For example, Flavobacteriales abundance was correlated with symptom development.
植物组织被称为内生菌的各种微生物群落所定殖。它们是植物产量和健康的关键决定因素，例如通过促进养分交换或限制疾病发展。直到最近才开始研究香蕉植物的内生菌群落，到目前为止尚未探索它们在疾病发展中的潜在作用。从受f.sp. 小种1感染的田间采集有症状和无症状香蕉植物的根。目的是通过16s rDNA高通量测序和鸟枪法宏基因组测序比较有症状和无症状植物之间的内生微生物群。结果表明，香蕉内生根微生物群以变形菌门和拟杆菌门为主，放线菌门的占比相对较小。疾病的发展极大地影响了内生微生物群落。例如，黄杆菌目丰度与症状发展相关。
