Key Lab of Horticultural Plant Biology, Ministry of Education, and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Lab of Horticultural Plant Biology, Ministry of Education, and College of Plant Science and Technology, Huazhong Agricultural University; and Pesticides Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
Plant Dis. 2018 Jul;102(7):1299-1306. doi: 10.1094/PDIS-10-17-1531-RE. Epub 2018 Apr 26.
Altogether, 192 Botrytis cinerea isolates collected from tomato greenhouses at different locations in Hubei Province were evaluated for their sensitivity to fungicides procymidone and zoxamide. The mean effective concentration to cause 50% growth inhibition (EC) values of procymidone for sensitive and resistant isolates were 0.25 and 3.60 μg/ml, respectively. The frequency of procymidone-resistant (Pro) isolates was 18%, and the highest frequency was recorded in Jingmen. Positive cross-resistance was observed for Pro isolates to other dicarboximide fungicides but not to phenylpyrroles. Significant differences were observed for fitness parameters (i.e., mycelial growth, osmotic sensitivity, and virulence between sensitive and resistant isolates). Amino acid sequence of the Bos1 gene revealed that Pro isolates carried either point mutations at codon 365 (I365S) or a pair of point mutations at codons 369 (Q369P) and 373 (N373S). For zoxamide, the mean EC values for sensitive and resistant isolates were 0.22 and 5.32 μg/ml, respectively. Approximately 14% of the isolates were found to be resistant to zoxamide, and the highest frequency of resistance was also observed in Jingmen. There was positive cross-resistance for zoxamide-resistant (Zox) isolates to carbendazim. No significant differences were observed for fitness parameters between zoxamide-sensitive and Zox isolates. Sequence analysis of the β-tubulin gene of Botrytis cinerea revealed two previously reported point mutations (E198A and E198K) and one new point mutation (T351I). This new mutation was detected in only those isolates which possessed the E198K but not E198A substitution. This study allows for a better understanding of the resistance development profile in Hubei Province. Results will be useful for the improvement of fungicide resistance management strategies.
总共从湖北省不同地点的番茄温室中采集了 192 株灰葡萄孢菌分离物，评估了它们对杀菌剂腐霉利和唑酰胺的敏感性。敏感和抗性分离物对腐霉利的平均有效浓度（EC）值分别为 0.25 和 3.60μg/ml。腐霉利抗性（Pro）分离物的频率为 18%，荆门记录的频率最高。Pro 分离物对其他二羧酰胺类杀菌剂表现出正交叉抗性，但对苯并吡咯类杀菌剂没有表现出正交叉抗性。在敏感和抗性分离物之间，观察到了适应性参数（即菌丝生长、渗透压敏感性和毒力）的显著差异。Bos1 基因的氨基酸序列表明，Pro 分离物要么在密码子 365 处发生点突变（I365S），要么在密码子 369（Q369P）和 373（N373S）处发生一对点突变。对于唑酰胺，敏感和抗性分离物的平均 EC 值分别为 0.22 和 5.32μg/ml。约 14%的分离物对唑酰胺表现出抗性，荆门的抗性频率最高。唑酰胺抗性（Zox）分离物对多菌灵表现出正交叉抗性。唑酰胺敏感和 Zox 分离物之间的适应性参数没有显著差异。灰葡萄孢β-微管蛋白基因序列分析显示了两个先前报道的点突变（E198A 和 E198K）和一个新的点突变（T351I）。仅在那些具有 E198K 而不是 E198A 取代的分离物中检测到该新突变。本研究有助于更好地了解湖北省的抗性发展情况。研究结果将有助于改进杀菌剂抗性管理策略。
