Zhao Haipeng, Ding Xiangyu, Chu Xiaomeng, Zhang Haimiao, Wang Xinyu, Zhang Xinwen, Liu Haoqi, Zhang Xiaoying, Yin Ziyi, Li Yang, Ding Xinhua
State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, Shandong, P. R. China.
Shandong Pengbo Biotechnology Co., Ltd., Taian, 271000, China.
Stress Biol. 2023 Aug 22;3(1):36. doi: 10.1007/s44154-023-00106-0.
Gray mold is a destructive disease caused by Botrytis cinerea, a pervasive plant pathogen, which poses a threat to both tomato growth and postharvest storage. The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks. ZNC (zhinengcong), an extract derived from the endophytic fungus Paecilomyces variotii, has been discovered to play a vital role in preventing diverse forms of bacterial infections. Nevertheless, the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear. In this study, we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B. cinerea. The results of both the metabolomic analysis and high-performance liquid chromatography (HPLC) demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin. Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species (ROS) and phosphorylation of mitogen-activated protein kinases (MPKs) related genes expression during the initial phase of invasion by B. cinerea. In addition, we also found that rutin might activate plant immunity by eliciting ethylene (ET) and jasmonic acid (JA)-mediated pathways. Therefore, plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold.
灰霉病是由灰葡萄孢引起的一种毁灭性病害，灰葡萄孢是一种普遍存在的植物病原体，对番茄生长和采后储存均构成威胁。利用诱导抗性是对抗植物病原体攻击的一种潜在策略。智能聪（ZNC）是一种从内生真菌拟青霉中提取的提取物，已发现其在预防多种形式的细菌感染中发挥着重要作用。然而，其增强番茄对真菌抗性的具体机制尚不清楚。在本研究中，我们发现外源喷施ZNC可显著提高番茄植株对灰葡萄孢的抗性。代谢组学分析和高效液相色谱（HPLC）结果均表明，番茄植株通过积累高水平的芦丁对ZNC处理作出反应。进一步的转录组分析发现，芦丁可能通过在灰葡萄孢入侵初期引发活性氧（ROS）的产生以及与丝裂原活化蛋白激酶（MPK）相关基因表达的磷酸化来增强番茄抗性。此外，我们还发现芦丁可能通过引发乙烯（ET）和茉莉酸（JA）介导的途径来激活植物免疫。因此，植物免疫诱导剂ZNC和芦丁在防治灰霉病方面具有广阔的应用前景和较高的利用价值。
