Sarven Most Sinthia, Hao Qiuyan, Deng Junbo, Yang Fang, Wang Gaofeng, Xiao Yannong, Xiao Xueqiong
Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Jingmen (China Valley) Academy of Agricultural Science, Jingmen 448000, Hubei, China.
Pathogens. 2020 Mar 13;9(3):213. doi: 10.3390/pathogens9030213.
Gray mold disease caused by is a devastating disease that leads to serious financial loss. In this study, the entomopathogenic fungus that acts against the gray mold pathogen was evaluated. produced a significant inhibition zone in front of the colony in the dual culture test. In addition, volatile organic compounds generated by were shown to have an inhibitory effect on mycelia growth and reduced 41% of gray mold severity of postharvest tomatoes. The 10% concentration of the culture filtrate of inhibited 88.62% of colony radial growth as well as 63.85% of sclerotia germination and all conidia germination of . Furthermore, the culture filtrate of retained its inhibitory effect against the radial growth of even after heating for 15 min at 100 °C. Feasible mechanisms of involved in the control of were explored, and it was demonstrated that the plasma membrane of conidia was damaged by the product of metabolism of . In addition, after treating with culture filtrate of , the phenotype was shown to be abnormal, and cell organelles of mycelia were damaged significantly. A significant control efficacy of against tomato gray mold was detected on both the detached leaf assay (84.24%) as well as the whole plant (72.38%). In addition, a 78% reduction in tomato fruit mold was detected at a 10% treated concentration of . These findings suggest that possesses potential as a biocontrol agent against tomato gray mold in the greenhouse and during the postharvest stage.
由[未提及具体病原菌名称]引起的灰霉病是一种毁灭性病害，会导致严重的经济损失。在本研究中，对一种针对灰霉病病原菌[未提及具体病原菌名称]的昆虫病原真菌[未提及具体真菌名称]进行了评估。在双培养试验中，[未提及具体真菌名称]在[未提及具体病原菌名称]菌落前产生了明显的抑菌圈。此外，[未提及具体真菌名称]产生的挥发性有机化合物对[未提及具体病原菌名称]菌丝体生长具有抑制作用，并使采后番茄的灰霉病严重程度降低了41%。[未提及具体真菌名称]培养滤液的10%浓度抑制了[未提及具体病原菌名称]菌落径向生长的88.62%，以及菌核萌发的63.85%和所有分生孢子萌发。此外，[未提及具体真菌名称]的培养滤液即使在100℃加热15分钟后，对[未提及具体病原菌名称]的径向生长仍保持抑制作用。探讨了[未提及具体真菌名称]控制[未提及具体病原菌名称]的可能机制，结果表明[未提及具体病原菌名称]分生孢子的质膜被[未提及具体真菌名称]的代谢产物破坏。此外，用[未提及具体真菌名称]的培养滤液处理后，[未提及具体病原菌名称]的表型显示异常，[未提及具体病原菌名称]菌丝体的细胞器受到显著损伤。在离体叶片试验（84.24%）和整株植物试验（72.38%）中均检测到[未提及具体真菌名称]对番茄灰霉病具有显著的防治效果。此外，在10%的处理浓度下，检测到番茄果实霉烂减少了78%。这些发现表明，[未提及具体真菌名称]在温室和采后阶段具有作为番茄灰霉病生物防治剂的潜力。
