Park Hye Hun, Srisombat La-Ongnuan, Jamison Andrew C, Liu Tingting, Marquez Maria D, Park Hansoo, Lee Sungbae, Lee Tai-Chou, Lee T Randall
Department of Chemistry and the Texas Center for Superconductivity, University of Houston, Houston, TX 77204-5003, USA.
School of Integrative Engineering, Chung-Ang University, Seoul 156-756, Korea.
Gels. 2018 Mar 26;4(2):28. doi: 10.3390/gels4020028.
Gold nanoshells (~160 nm in diameter) were encapsulated within a shell of temperature-responsive poly(-isopropylacrylamide--acrylic acid) (P(NIPAM--AA)) using a surface-bound rationally-designed free radical initiator in water for the development of a photothermally-induced drug-delivery system. The morphologies of the resultant hydrogel-coated nanoshells were analyzed by scanning electron microscopy (SEM), while the temperature-responsive behavior of the nanoparticles was characterized by dynamic light scattering (DLS). The diameter of the P(NIPAM--AA) encapsulated nanoshells decreased as the solution temperature was increased, indicating a collapse of the hydrogel layer with increasing temperatures. In addition, the optical properties of the composite nanoshells were studied by UV-visible spectroscopy. The surface plasmon resonance (SPR) peak of the hydrogel-coated nanoshells appeared at ~800 nm, which lies within the tissue-transparent range that is important for biomedical applications. Furthermore, the periphery of the particles was conjugated with the model protein avidin to modify the hydrogel-coated nanoshells with a fluorescent-tagged biotin, biotin-4-fluorescein (biotin-4-FITC), for colorimetric imaging/monitoring.
使用一种表面结合的合理设计的自由基引发剂，在水中将直径约160纳米的金纳米壳包裹在温度响应性聚（N-异丙基丙烯酰胺-丙烯酸）（P（NIPAM-AA））壳内，以开发一种光热诱导药物递送系统。通过扫描电子显微镜（SEM）分析所得水凝胶包覆纳米壳的形态，同时通过动态光散射（DLS）表征纳米颗粒的温度响应行为。随着溶液温度升高，P（NIPAM-AA）包覆纳米壳的直径减小，表明水凝胶层随温度升高而塌陷。此外，通过紫外可见光谱研究了复合纳米壳的光学性质。水凝胶包覆纳米壳的表面等离子体共振（SPR）峰出现在约800纳米处，该波长位于对生物医学应用很重要的组织透明范围内。此外，颗粒的外围与模型蛋白抗生物素蛋白共轭，用荧光标记的生物素生物素-4-荧光素（生物素-4-FITC）修饰水凝胶包覆纳米壳，用于比色成像/监测。
